Anti-Neurofibromin C Antibody, clone NFC

32160702

Neurofibromin (UniProt: P21359; also known as Neurofibromatosis-related protein NF-1) is encoded by the NF1 gene (Gene ID: 4763) in human. Neurofibromin is a GTPase-activating protein that negatively regulatres RAS/MAOK pathway by enhancing the hydrolysis of Ras-bound GTP. Hence, it can display tumor suppressor properties. Neurofibromin has been detected in brain, peripheral nerves, lung, colon, and muscle tissues. It has a CRAL-TRIO domain (aa 1580-1738) that facilitates its binding to phospholipids. It binds primarily glycerophospholipids with monounsaturated C18:1 and/or C16:1 fatty acid moieties and a phosphatidylethanolamine or phosphatidylcholine head group. Neurofibromin also has a Ras-GTP domain (aa 1235-1451). Mutations in NF1 gene are known to cause Neurofibromatosis type I that involved tumors in the peripheral nervous system and fibromatous skin tumors. Some mutations are also known to cause colorectal cancer and juvenile leukemia. Six different isoforms of Neurofibromin have been described that are produced by alternative splicing. (Ref.: Rossi, S., et al. (2018). Mod. Pathol. 31(1), 160-168).

Clone NFC specifically detects human Neurofibromin C. it targets an epitope with in the C-terminal region.

Recombinant fragment corresponding to 281 amino acids from the C-terminal region of human Neurofibromin C.

Epitope: C-terminus

Anti-Neurofibromin C, clone NFC, Cat. No. MABE1820, is a highly specific mouse monoclonal antibody that targets Neurofibromin and has been tested for use in Immunohistochemistry (Paraffin).
Immunohistochemistry (Paraffin) Analysis: A representative lot detected Neurofibromin C in Immunohistochemistry applications (Rossi, S., et. al. (2018). Mod Pathol. 31(1):160-168).
Western Blotting Analysis: A representative lot detected Neurofibromin C lysate from human NF1 +/+ cells, but not in -/- cells or NF1+/+ cells transfected with siRNA targeting neurofibromin (Reuss, DE.,et al., (2014). Acta Neuropathol. 127(4); 565-572).
Research Category
Epigenetics & Nuclear Function

Evaluated by Immunohistochemistry (Paraffin) in human kidney tissue sections.

Immunohistochemistry (Paraffin) Analysis: A 1:50 dilution of this antibody detected Neurofibromin C in human kidney tissue sections.

Protein G purified
Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Format: Purified

Concentration: Please refer to lot specific datasheet.

mouse

purified immunoglobulin

primary antibodies

NFC, monoclonal

human

antibody small pack of 25 μL

immunohistochemistry: suitable (paraffin)

IgG1κ

P21359

human ... NF1(4763)

319.37 kDa calculated.

Stable for 1 year at 2-8°C from date of receipt.

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