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Anti-p53 (Ab-3) (Mutant) Mouse mAb (PAb240)
产品性质
Quality Level【质量水平】 | 100 |
biological source【生物来源】 | mouse |
antibody form【抗体形式】 | purified antibody |
antibody product type | primary antibodies |
clone【克隆】 | PAb240, monoclonal |
form【形式】 | liquid |
contains【包含】 | ≤0.1% sodium azide as preservative |
species reactivity | hamster, chicken, mouse, bovine, human, rat |
should not react with | Xenopus |
manufacturer/tradename | Calbiochem® |
storage condition【储存条件】 | do not freeze |
isotype【同位素/亚型】 | IgG1 |
shipped in【运输】 | wet ice |
storage temp.【储存温度】 | 2-8℃ |
packaging【包装】 | 100, 200 μg in Plastic ampoule Please refer to vial label for lot-specific concentration. |
基本信息
General description【一般描述】 | This Anti-p53 (Ab-3) (Mutant) Mouse mAb (PAb240) is validated for use in FC, Frozen Sections, Gel Shift, Immunoblotting, IF, IP, Paraffin Sections for the detection of p53 (Ab-3) (Mutant). Purified mouse monoclonal antibody generated by immunizing BALB/c mice with the specified immunogen and fusing splenocytes with SP2 mouse myeloma cells (see application references). Recognizes the ~53 kDa mutant p53 protein under non-denaturing conditions. Recognizes both the mutant and the wild-type p53 protein under denaturing conditions. Recognizes the ~53 kDa mutant p53 protein under non-denaturing conditions by immunoprecipitation, immunofluorescence, and flow cytometry. Recognizes both mutant and wild-type p53 by immunoblotting and paraffin sections under denaturing conditions. |
Immunogen【免疫原】 | Human Epitope: within amino acids 213-217 a recombinant protein consisting of amino acids 14-389 of p53 fused to β-galactosidase |
Application【应用】 | Flow Cytometry (1-20 µg/ml) Frozen Sections (10 µg/ml) Gel Shift (see comments) Immunoblotting (5 µg/ml) Immunofluorescence (1-20 µg/ml, see application references) Immunoprecipitation (1 µg per sample) Paraffin Sections (see application references) |
Warning【警告】 | Toxicity: Standard Handling (A) |
Physical form【外形】 | In 50 mM sodium phosphate buffer, 0.2% gelatin, pH 7.5. |
Analysis Note【分析说明】 | Negative Control SK-OV-3 cells Positive Control A431, Hs27 (wild-type p53), or SK-BR-3 cells or breast carcinoma tissue |
Other Notes【其他说明】 | El-Deiry, W.S., et al. 1994. Cancer Res.54, 1169. Greenblatt, M.S., et al. 1994. Cancer Res.54, 4855. Barak, Y., et al. 1993. EMBO J.12, 461. Kastan, M.B., et al. 1992. Cell71, 587. Kuerbitz, S.J. 1992. Proc. Natl. Acad. Sci. USA89, 7491. Lane, D.P. 1992. Nature358, 15. Kastan, M.B., et al. 1991. Cancer Res.51, 6304. Under non-denaturing conditions (immunoprecipitation, immunofluorescence and frozen sections), Anti-p53 (Ab-3) does not recognize normal (wild-type) p53 protein; it recognizes an epitope exposed by activating mutations or denaturation. In denaturing protocols (immunoblotting and paraffin sections), Anti-p53 (Ab-3) will recognize both mutant and wild-type p53. Will not recognize to some p53 molecules with mutations in the RHSVV epitope, but will react to TFIIIA, which has the RHSVV epitope. For gel shift assay, use Cat. No. OP29L and resuspend in 100 µl buffer. Antibody should be titrated for optimal results in individual systems. |
Legal Information【法律信息】 | CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany |
安全信息
Storage Class Code【储存分类代码】 | 12 - Non Combustible Liquids |
WGK | nwg |