Anti-PCNA (Ab-1) Mouse mAb (PC10)

100

mouse

purified antibody

primary antibodies

PC10, monoclonal

liquid

≤0.1% sodium azide as preservative

mouse, rat, yeast, human, zebrafish

Calbiochem^®

do not freeze

IgG2a

wet ice

2-8℃

200 μg in Plastic ampoule
Please refer to vial label for lot-specific concentration.

Recognizes the ~37 kDa PCNA protein in HeLa cells as well as in tonsil or normal colon tissue.
Purified mouse monoclonal antibody generated by immunizing BALB/c mice with the specified immunogen and fusing splenocytes with SP2/0-Ag14 mouse myeloma cells. Recognizes the ~37 kDa PCNA protein.
This Anti-PCNA (Ab-1) Mouse mAb (PC10) is validated for use in Immunoblotting, Immunofluorescence, Immunoprecipitation Paraffin Sections for the detection of PCNA (Ab-1).

Epitope: Within amino acids 112-121
recombinant PCNA

Immunoblotting (2.5 µg/ml)

Immunofluorescence (1-5 µg/ml)

Immunoprecipitation (not recommended with unconjugated antibody, see comments)

Paraffin Sections (2.5 µg/ml, no pre-treatment required, see comments)

Toxicity: Standard Handling (A)

Positive Control
HeLa cells, HL-60 cells, tonsil tissue, or normal colon tissue

Waseem, N.H. and Lane, D.P. 1990. J. Cell. Sci.96, 121.
Suzuka, I., et al. 1989. Proc. Natl. Acad. Sci. USA86 3189.
Bravo, R., et al. 1987. Nature (London)326, 515.
Bravo, R. and MacDonald-Bravo, H. 1987. J. Cell. Biol.105, 1549.
Miyachi, K., et al. 1987. J. Immunol.121, 2228.
Staining shows an intense nuclear pattern. Not recommended for immunoprecipitation because in some samples a ~37 kDa protein will be precipitated that is not PCNA. Generally, no pre-treatment is needed to stain paraffin sections, but staining may be enhanced in some samples by pretreating with 4 N HCl for 10 min at 37°C followed by 3 washes in water. Antibody should be titrated for optimal results in individual systems.

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12 - Non Combustible Liquids

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