Anti-PGM5 (Aciculin) Antibody, clone 14F8

32160702

NA.41

Phosphoglucomutase-like protein 5 (UniProt: Q15124; also known as Aciculin, Phosphoglucomutase-related protein, PGM-RP) is encoded by the PGM5 (also known as PGMRP) gene (Gene ID: 5239) in human. Aciculin is a dystrophin and utrophin-binding protein that is highly homologous to the glycolytic enzyme phosphoglucomutase type 1. However, it lacks phosphoglucomutase activity. Aciculin is a component of adherens-type cell-cell and cell-matrix junctions and is concentrated in focal contacts at the ends of actin bundles and associates with actin filaments. Aciculin is also reported to be an interaction partner of Filamin C and Xin proteins. Higher levels of Aciculin are reported in smooth and cardiac muscle and lower levels are present in skeletal muscle, liver, kidney and brain tissue. In striated muscle, it mainly localizes to the intercalated discs of the heart and to myotendinous junctions. Low levels of Aciculin are expressed in 10-12-week-old fetus and these levels increase progressively into infancy reaching maximum levels at maturity. Two isoforms of Aciculin have been reported that are produced by alternative splicing. Aciculin knockdown in myotubes is shown to cause failure in myofibril assembly, alignment and membrane attachment, and a massive reduction in myofibril number. (Ref.: Molt, S., et al., (2014). J. Cell Sci.127(16); 3578-3592).

Clone 14F8 detects Phosphoglucomutase-like protein 5 in mouse and rat cardiac tissue and is predicted to react with human.

Cytoskeletal fraction of the human myometrium tissue extract.

Research Category
Cell Structure
Western Blotting Analysis: 1 µg/mL from a representative lot detected PGM5 (Aciculin) in rat heart tissue lysate.

Immunoprecipitation Analysis: A representative lot immunoprecipitated PGM5 (Aciculin) in Immunoprecipitation applications (Belkin, A.M., et. al. (1995). J Biol Chem. 270(11):6328-37; Belkin, A.M., et. al. (1995). Exp Cell Res. 221(1):132-40).

Immunofluorescence Analysis: A representative lot detected PGM5 (Aciculin) in Immunofluorescence applications (Belkin, A.M., et. al. (1994). J Cell Sci. 107 ( Pt 7):1993-2003).

Immunohistochemistry Analysis: A representative lot detected PGM5 (Aciculin) in Immunohistochemistry applications (Belkin, A.M., et. al. (1995). Exp Cell Res. 221(1):132-40).

Western Blotting Analysis: A representative lot detected PGM5 (Aciculin) in Western Blotting applications (Belkin, A.M., et. al. (1995). J Biol Chem. 270(11):6328-37; Belkin, A.M., et. al. (1994). J Cell Sci. 107 ( Pt 7):1993-2003).

ELISA Analysis: A representative lot detected PGM5 (Aciculin) in ELISA applications (Molt, S., et. al. (2014). J Cell Sci. 127(Pt 16):3578-92).

Anti-PGM5 (Aciculin), clone 14F8, Cat. No. MABT1503, is a mouse monoclonal antibody that detects Phosphoglucomutase-like protein 5 and has been tested for use in ELISA, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, and Western Blotting.

Evaluated by Western Blotting in mouse heart tissue lysate.

Western Blotting Analysis: 1 µg/mL of this antibody detected PGM5 (Aciculin) in mouse heart tissue lysate.

Protein G purified
Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Format: Purified

Concentration: Please refer to lot specific datasheet.

mouse

purified antibody

primary antibodies

14F8, monoclonal

human, rat, rabbit, bovine, mouse

porcine, frog, avian

antibody small pack of 25 μg

ELISA: suitable
immunofluorescence: suitable
immunohistochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable

IgG1κ

NP_068800

Q15124

human ... PGM5(5239)

~57 kDa observed; 62.22 kDa calculated. Uncharacterized bands may be observed in some lysate(s).

Stable for 1 year at 2-8°C from date of receipt.

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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