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Anti-TIMP-1 (Ab-1) Mouse mAb (7-6C1)
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产品性质
Quality Level【质量水平】 | 100 |
biological source【生物来源】 | mouse |
antibody form【抗体形式】 | purified antibody |
antibody product type | primary antibodies |
clone【克隆】 | 7-6C1, monoclonal |
form【形式】 | liquid |
contains【包含】 | ≤0.1% sodium azide as preservative |
species reactivity | human, rat, bovine |
should not react with | mouse |
manufacturer/tradename | Calbiochem® |
storage condition【储存条件】 | OK to freeze avoid repeated freeze/thaw cycles |
isotype【同位素/亚型】 | IgG1 |
shipped in【运输】 | wet ice |
storage temp.【储存温度】 | −20℃ |
packaging【包装】 | 100 μg in Plastic ampoule Please refer to vial label for lot-specific concentration. |
基本信息
General description【一般描述】 | Purified mouse monoclonal antibody (see application references). Recognizes the ~28 kDa TIMP-1 protein. Matrix metalloproteinases (MMPs) are a family of enzymes that are responsible for the degradation of extracellular matrix components such as collagen, laminin and proteoglycans. In addition to sequence homology, all MMPs share the following characteristics: the catalytic mechanism is dependent upon a zinc ion at the active center, they cleave one or more extracellular matrix components, they are secreted as zymogens which are activated by removal of an approximately 10 kDa segment from the N-terminus and their activity is regulated by endogenous inhibitors. These enzymes are involved in normal physiological processes such as embryogenesis and tissue remodeling and may play an important role in arthritis, periodontitis, and metastasis.The activation and activity of MMPs are regulated by a family of endogenous inhibitors, tissue inhibitors of metalloproteinase (TIMP). TIMP-1 (also called EPA, Fibroblast collagenase inhibitor or collagenase inhibitor) is a 28 kDa glycoprotein that is expressed by a variety of cell types. It forms a non-covalent, stoichiometric complex with both latent and active MMPs. TIMP-1 preferentially binds and inhibits MMP-9. TIMPs are capable of altering the metastatic potential of cancer cells and have been shown to inhibit invasion and metastasis in animal models. This Anti-TIMP-1 (Ab-1) Mouse mAb (7-6C1) is validated for use in Immunoblotting, Immunocytochemistry, Paraffin Sections for the detection of TIMP-1 (Ab-1). Recognizes the ~28.5 kDa TIMP-1 protein. Also recognizes MMP/TIMP-1 complexes. |
Immunogen【免疫原】 | Bovine purified, bovine dental pulp TIMP-1 |
Application【应用】 | ![]() Immunoblotting (1 g/ml, see application references) Immunocytochemistry (see application references) Paraffin Sections (25 g/ml, see application references, see comments) |
Warning【警告】 | Toxicity: Standard Handling (A) |
Physical form【外形】 | In 100 mM sodium phosphate buffer, 0.1% BSA, pH 7.0. |
Reconstitution【重悬】 | Following initial thaw, aliquot and freeze (-20°C). |
Analysis Note【分析说明】 | Positive Control rTIMP-1 Protein (Cat. Nos. PF019 or PF020) |
Other Notes【其他说明】 | Fujimoto, N. and Iwata, K., 1994. Connective Tissue26, 237. Cottam, D. W. and Rees, R. C., 1993. Intl. J. Oncol.2, 861. Stetler-Stevenson, W. G., et al. 1993. FASEB J.7, 1434. Woessner, J. F., 1991. FASEB J.5, 2145. Liotta, L. A. and Stetler-Stevenson, W. G. 1990. in Seminars in Cancer Biology ed. M. M. Gottesman. Vol. 99-106. Also recognizes TIMP-1/MMP complexes.This antibody has been used to stain paraffin sections fixed with PLP (perioddate-lysine-4% paraformaldehyde) and 10% formaldehyde (using 25 µg/ml antibody). Antibody should be titrated for optimal results in individual systems. |
Legal Information【法律信息】 | Manufactured by Daiichi Fine Chemical Co., Ltd. Not available for sale in Japan. CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany |