Anti-Tspan-8 Antibody, clone 40/15-1B11-63-76-8

32160702

Tetraspanin-8 (UniProt: Q8R3G9; also known as Tspan8, Tspan-8) is encoded by the Tspan8 gene (Gene ID: 216350) in murine species. Tspan-8 is a multi-pass membrane protein of the tetraspanin family that is involved in the regulation of cell activation, growth, and motility. Members of this family have emerged as important regulators of expression levels, trafficking or post-translational modification of laminin-binding integrins. Tetraspanins are shown to associate with one another and cluster dynamically with a large variety of transmembrane and signal-transducing partners, forming specialized membrane microdomains called tetraspanin web. Tspan-8 is reported to be overexpressed during the progression of colorectal, liver, pancreatic and gastric cancers and its increased expression is shown to promote liver and lung metastasis. Overexpression of Tspan-8 has also been correlated with poor prognosis in human colorectal cancer. In metastatic pancreatic and colorectal carcinoma cell lines, PKC activation enhances the colocalization of Tspan-8 and Tspan CD151 with integrin alpha6beta 4 and subsequent internalization of this integrin-tetraspanin complex, resulting in decreased cell-matrix adhesion and increased cell migration. Tspan-8 knockdown in HT29 colon cancer cells is reported to significantly reduce cell migratory ability as a result of an upregulation of both integrin-dependent cell-matrix adhesion and calcium-dependent cell-cell adhesion. (Ref.: Kharbili, ME., et al. (2017). Oncotarget. 8(10); 17140-17155).

Clone 40/15-1B11-63-76-8 is a rat monoclonal antibody that detects Tetraspanin-8. It targets an epitope with in the extracellular domain.

KLH-conjugated linear peptide corresponding to 10 amino acids from the extracellular domain of murine Tetraspanin-8.

Epitope: extracellular domain

Research Category
Cell Structure
Anti-Tspan-8, clone 40/15-1B11-63-76-8 (Anti-Tspan8-A), Cat. No. MABT1332, is a rat monoclonal antibody that detects Tetraspanin-8 and has been tested for use in Immunohistochemistry (Paraffin) and Western Blotting.
Immunohistochemistry Analysis: A representative lot detected Tspan-8 in Immunohistochemistry applications (Fu, N.Y., et. al. (2017). Nat Cell Biol. 19(3):164-176).

Western Blotting Analysis: 4 µg/mL from a representative lot detected Tspan-8 in LIM1215 positive for Tspan8 and HEK293T cell lysates.

Evaluated by Immunohistochemistry (Paraffin) in mouse colon tissue sections.

Immunohistochemistry (Paraffin) Analysis: A 1:50 dilution of this antibody detected Tspan-8 in mouse colon tissue sections.

Protein G purified
Format: Purified
Purified rat monoclonal antibod yIgG2a in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Concentration: Please refer to lot specific datasheet.

rat

purified immunoglobulin

primary antibodies

40/15-1B11-63-76-8, monoclonal

mouse

human (based on 100% sequence homology), monkey (based on 100% sequence homology)

antibody small pack of 25 μg

immunohistochemistry: suitable (paraffin)
western blot: suitable

IgG2aκ

NP_666122.1

Q8R3G9

25.58 kDa calculated.

Stable for 1 year at 2-8°C from date of receipt.

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.