泛素小鼠单克隆抗体(FK2)

100

mouse

unpurified

primary antibodies

FK2, monoclonal

liquid

≤0.1% sodium azide as preservative

all

Calbiochem^®

OK to freeze
avoid repeated freeze/thaw cycles

IgG1

wet ice

−20℃

100 μg in Glass bottle
Please refer to vial label for lot-specific concentration.

This Anti-Ubiquitin Mouse mAb (FK2) is validated for use in ELISA, Immunoblotting, Immunoprecipitation, Immunofluorescence for the detection of Ubiquitin.
Recognizes K²⁹-, K⁴⁸-, and K⁶³-linked polyubiquitinylated and monoubiquitinylated proteins, but not free ubiquitin, in Raji cells.
Mouse monoclonal antibody generated by immunizing Balb/c mice with the specified immunogen and fusing splenocytes with myeloma cells. Recognizes K²⁹-, K⁴⁸-, and K⁶³-linked polyubiquitinylated and monoubiquitinylated proteins.

unpurified polyubiquitinylated lysozyme
human

ELISA (see application references)
Immunoblotting (1:1000)
Immunoprecipitation (see application references and comments)
Immunofluorescence (see application references)

Toxicity: Standard Handling (A)

In PBS.

Raji cells

Following initial thaw, aliquot and freeze (-20°C). Store diluted antibody at 4°C for up to 1 month.

Matsumoto, M., et al. 2005. Proteomics 5, 4545.
Boname, J.N., et al. 2001. Immunity 15, 627.
Campbell, V., et al. 2001. Neuron 32, 1013.
Fujimuro, M., et al. 2003. FEBS Letts. 349, 173.
Does not recognize free ubiquitin. For immunoprecipitation, once the antibody is immobilized, it demonstrates affinity for free ubiquitin as well as for poly- and monoubiquitinylated proteins. Antibody should be titrated for optimal results in individual systems.

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

12 - Non Combustible Liquids

WGK 2