Anti-UGT1A10 Antibody, clone 20H1

32160702

UDP-glucuronosyltransferase 1-10 (UniProt: Q9HAW8; also known as EC: 2.4.1.17, UDPGT 1-10, UGT1*10, UGT1-10, UGT1.10, UDP-glucuronosyltransferase 1-J, UGT-1J, UGT1J, UDP-glucuronosyltransferase 1A10) is encoded by the UGT1A10 (also known as GNT1, UGT1) gene (Gene ID: 54575) in human. UDP-Glucuronosyltransferases (UGTs) are a major group of phase II drug metabolizing enzymes and each enzyme in this family displays a unique substrate specificity and tissue-specific expression patterns. UDP-Glucuronosyltransferases mediate the transfer of glucuronic acid from UDP-glucuronic acid to various aglycone substrates making them more hydrophilic and readily excretable. UGT1A10 is a single-pass membrane protein that is synthesized with a signal peptide (aa 1029), which is subsequently cleaved off in the mature form. Two isoforms of UGT1A10 have been described that are produced by alternative splicing. Both isoforms 1 and 2 are expressed in colon, esophagus, and small intestine. However, in liver only isoform 2 is expressed. Isoform 2 lacks transferase activity, but it can act as a negative regulator of isoform 1. Expression of UGT1A10 is regulated both epigenetically and transcriptionally in response to oxidative stress and aryl hydrocarbon receptor agonists. Clone 20H1 is highly specific for UGT1A10 that can also detect differentially glycosylated forms of UGT1A10. (Ref.: Oda, S., et al. (2017). Drug Metab. Dispos. 45:1027-1034).

Clone 20H1 specifically detects human UDP-glucuronosyltransferase 1-10. It targets an epitope with in 32 amino acids from the N-terminal region.

KLH-conjugated linear peptide corresponding to 32 amino acids from the N-terminal region of human UDP-glucuronosyltransferase 1-10.

Anti-UGT1A10, clone 20H1, Cat. No. MABS2070, is a mouse monoclonal antibody that detects UDP-glucuronosyltransferase 1-10 and has been tested for use in Immunohistochemistry (Paraffin) and Western Blotting.
Western Blotting Analysis: A representative lot detected UGT1A10 in Western Blotting applications (Oda, S., et. al. (2017). Drug Metab Dispos. 45(9):1027-1034).

Immunohistochemistry (Paraffin) Analysis: A representative lot detected UGT1A10 in Immunohistochemistry applications (Oda, S., et. al. (2017). Drug Metab Dispos. 45(9):1027-1034).

Research Category
Signaling

Evaluated by Immunohistochemistry (Paraffin) in human small intestine tissue sections.

Immunohistochemistry (Paraffin) Analysis: A 1:50 dilution of this antibody detected UGT1A10 in human small intestine tissue sections.

Protein G purified
Format: Purified
Purified mouse monoclonal antibody IgG3 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Concentration: Please refer to lot specific datasheet.

mouse

purified immunoglobulin

primary antibodies

20H1, monoclonal

human

antibody small pack of 25 μL

immunohistochemistry: suitable (paraffin)
western blot: suitable

IgG3κ

NP_061948.1

Q9HAW8

59.81 kDa calculated.

Stable for 1 year at 2-8°C from date of receipt.

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