Anti-Vitamin D Receptor (Ab-1) Rat mAb (9A7γE10.4)

100

rat

purified antibody

primary antibodies

9A7γE10.4, monoclonal

liquid

≤0.1% sodium azide as preservative

chicken, rat, porcine, mouse, amphibian, human, hamster

Calbiochem^®

OK to freeze
avoid repeated freeze/thaw cycles

IgG1

wet ice

2-8℃

100 μg in Plastic ampoule
Please refer to vial label for lot-specific concentration.

Purified rat monoclonal antibody generated by immunizing rats with the specified immunogen and fusing splenocytes with mouse myeloma p3 NS 1/Ag4-1 (NS1) mouse myeloma cells. Recognizes both the occupied and unoccupied forms of vitamin D receptor.
This Anti-Vitamin D Receptor (Ab-1) Rat mAb (9A7γE10.4) is validated for use in Frozen Sections, Gel Shift, WB, IP, Paraffin Sections for the detection of Vitamin D Receptor (Ab-1).
Recognizes both occupied and unoccupied vitamin D receptor in breast carcinomas expressing the vitamin D receptor protein.

purified, chick intestine vitamin D receptor

Frozen Sections (1:50-1:100)

Gel Shift (see comments)

Immunoblotting (1:200-1:400)

Immunoprecipitation (10 µl/mg protein lysate)

Paraffin Sections (1:50-1:100, sample pre-treatment required, see comments)

Toxicity: Standard Handling (A)

Positive Control
Breast carcinoma tissue (see comments)

Brown, A.J. 1999. Nephrol. Dial. Transplant.14, 11.
Fleet, J.C. 1999. Nutr. Rev.57 60.
Evans, S.R., et al. 1998. Clin. Cancer Res.4, 1591.
Friedrich, M., et al. 1998. J. Histochem. Cytochem.46, 1335.
Issa, L.L., et al. 1998. Inflamm. Res.47, 451.
Kivineva, M., et al. 1998. J. Steroid Biochem. Mol. Biol.66, 121.
Nangia, A.K., et al. 1998. J. Steroid Biochem. Mol. Biol.66, 241.
Does not cross-react with estrogen and glucocorticoid receptors. About 50% of breast tumors express vitamin D receptor protein. For gel shift assays, order antibody at 1 mg/ml; contact Customer Service for additional information. For paraffin sections pre-treatment in 2N HCl for 20 min at 37°C prior to staining. Antibody should be titrated for optimal results in individual systems.

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

11 - Combustible Solids

WGK 1