Endoglycosidase H, Streptomyces plicatus, Recombinant, E. coli

3.2.1.96 ( BRENDA | IUBMB )

Recombinant, streptomyces plicatus endoglycosidase H expressed in E. coli. Catalyzes the hydrolysis of asparagine-linked oligomannose and hybrid, but not complex oligosaccharides from glycoprotein. Endo H cleaves between the two N-acetylglucosamine residues in the diacetylchitobiose core of the oligosaccharide.
Note: 1 mU = 1 milliunit.

Toxicity: Standard Handling (A)

One unit is defined as the amount of enzyme that will catalyze the release of N-linked oligosaccharides from 1.0 µmol denatured ribonuclease B per min at 37°C, pH 5.5. Cleavage is monitored by SDS-PAGE.

In 50 mM NaCl, 20 mM Tris-HCl, 1 mM EDTA, pH 7.5.

Rao, V., et al. 1995. Structure 3, 449.
Trimble, R.B., et al. 1987. Methods Enzymol. 138, 763.
Trumbly, R.J., et al. 1985. J. Biol. Chem.260, 5683.

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

100

expressed in E. coli

liquid

≥3 units/mL
≥30 units/mg protein

Calbiochem^®

do not freeze

N-acetylglucosaminidase, α- and β-galactosidase, α-mannosidase, neuraminidases, proteases, none detected

wet ice

2-8℃

200 miu in Plastic ampoule

12 - Non Combustible Liquids

WGK 2