Histone Deacetylase 6, GST-Fusion, Human Recombinant, S. frugiperda

expressed in insect cells (S. frugiperda)

100

≥80% (SDS-PAGE)

liquid

≥50 units/μg protein

Calbiochem^®

OK to freeze
avoid repeated freeze/thaw cycles

wet ice

−70℃

50 μg in Plastic ampoule
Please refer to vial label for lot-specific concentration.

Full length, recombinant, human histone deacetylase 6 (HDAC6) fused to GST at the N-terminus and expressed in S. frugiperda insect cells using a baculovirus expression system. Actively maintained in the cytoplasm, HDAC6 is uniquely dependent on its ubiquitin-binding domain and functions at the nexus between two cellular signaling systems, involving protein acetylation and ubiquitination. The active deacetylase is useful in the study of HDAC6 regulation and for inhibitor screening.

Toxicity: Standard Handling (A)

One unit is defined as the amount of enyme that is required to deacetylate 1 pmol fluorescent HDAC peptide substrate per min at 30°C.

40 mM Tris-HCl pH8.0, 110mM NaCl, 2.2 mM KCl, 0.04% Tween 20, 3mM DTT, and 20% glycerol.

Following initial thaw, aliquot and freeze (-70°C).

Pandey, U.B., et al. 2007. Nature447, 859.
Hubbert, C., et al. 2002. Nature417, 455.

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

10 - Combustible liquids

WGK 1