Histone Deacetylase 8, His·Tag^®, Human Recombinant, S. frugiperda

expressed in insect cells (S. frugiperda)

100

≥90% (SDS-PAGE)

liquid

≥10 units/μg protein

Calbiochem^®

OK to freeze
avoid repeated freeze/thaw cycles

wet ice

−70℃

50 μg in Plastic ampoule
Please refer to vial label for lot-specific concentration.

Full length, recombinant, human histone deacetylase 8 (HDAC8) fused to at the C-terminus to a His•Tag sequence at and expressed in S. frugiperda insect cells using a baculovirus expression system. HDAC8 phosphorylated in vivo by PKA results in decreased activity, which leads to the hyperacetylation of histones H3 and H4. HDAC8 may play a role in one of the most frequent types of acute myeloid leukemia (AML). The active deacetylase is useful in the study of HDAC8 regulation and for inhibitor screening.

Toxicity: Standard Handling (A)

One unit is defined as the the amount of enzyme required to deacetylate 1 picomol fluorescent peptide substrate [Arg-His-Lys(Ac)-Lys(Ac)] per min at 30°C.

In 138 mM NaCl, 25 mM Tris-HCl, 10% glycerol, 0.05% TWEEN^®-20 detergent, pH 8.0.

Following initial thaw, aliquot and freeze (-70°C).

Waltregny, D., et al. 2005. FASEB J.19, 966.
Buggy, J.J., et al. 2000. Biochem J. 350, 199

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
HIS TAG is a registered trademark of Merck KGaA, Darmstadt, Germany
TWEEN is a registered trademark of Croda International PLC

10 - Combustible liquids

WGK 1