InSolution Smoothened 激动剂，SAG-Calbiochem

C₂₈H₂₈ClN₃OS · 2HCl · xH₂O

562.98 (anhydrous basis)

A cell-permeable benzothiophene compound that modulates the coupling of Smo with its downstream effector by interacting with the Smo heptahelical domain (K_D = 59 nM). Shown to induce Hedgehog pathway activation (EC₅₀ ∼3 nM in NIH 3T3-derived Shh-LIGHT2 cells) and counteracts Cyclopamine-KAAD (Cat. No. 239804) inhibition of Smo. Reported to act as an activator at low concentrations and as an inhibitor at very high concentrations.

Toxicity: Standard Handling (A)

A 10 mM (500 µg/86 µl) solution of Smoothened Agonist, SAG (Cat. No. 566660) in H₂O.

Following initial thaw, aliquot and freeze (-20°C).

Meloni, A.R., et al. 2006. Mol. Cell. Biol.26, 7750.
Chen, W., et al. 2004. Science306, 2257.
Chen, J.K., et al. 2002. Proc. Natl. Acad. Sci. USA99, 14071.
Frank-Kamenetsky, M., et al. 2002. J. Biol.1, 10.

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

100

≥95% (HPLC)

liquid

Calbiochem^®

OK to freeze
protect from light

wet ice

−20℃

500 μg in Plastic ampoule
Packaged under inert gas

12 - Non Combustible Liquids

WGK 2