PhosphoDetect Anti-Phosphotyrosine Mouse mAb (PY20)

100

mouse

purified antibody

primary antibodies

PY20, monoclonal

liquid

≤0.1% sodium azide as preservative

all

Calbiochem^®

OK to freeze
avoid repeated freeze/thaw cycles

IgG2b

wet ice

−20℃

100 μg in Plastic ampoule
Please refer to vial label for lot-specific concentration.

Recognizes a broad spectrum of tyrosine-phosphorylated proteins in their native and denatured states in crude extracts.
Purified mouse monoclonal antibody. Recognizes a broad spectrum of tyrosine-phosphorylated proteins in their native and denatured states.
This PhosphoDetect Anti-Phosphotyrosine Mouse mAb (PY20) is validated for use in ELISA, Immunoblotting, Frozen Sections, ICC, IP for the detection of Phosphotyrosine.

KLH conjugated to phosphotyrosine, glycine, and alanine

ELISA (see comments)

Immunoblotting (1:500-1:1000, see application references)

Frozen Sections (see application references)

Immunocytochemistry (see application references)

Immunoprecipitation (see comments)

Toxicity: Standard Handling (A)

In 10 mM sodium phosphate, pH 7.4.

Following initial thaw, aliquot and freeze (-20°C).

Positive Control
Any cell lysate or tissue extract

Glenney, J.R., et al. 1988. J. Immunol. Methods109, 277.
Binding is inhibited by phosphotyrosine. Does not react with threonine or serine phosphorylated residues on proteins. Do not use milk as a blocking agent with this antibody. Milk contains many phosphotyrosines and will adsorb the antibody. This antibody has also been reported to work for ELISA and immunoprecipitation. Variables associated with assay conditions will dictate the proper working dilution.

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

10 - Combustible liquids

WGK 1