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PhosphoDetect Anti-VASP (pSer157) Mouse mAb (5C6)
产品别名
PhosphoDetect Anti-VASP (pSer157) Mouse mAb (5C6)
Anti-Vasodilator-stimulated Phosphoprotein, Activated
产品性质
| Quality Level【质量水平】 | 100 |
| biological source【生物来源】 | mouse |
| antibody form【抗体形式】 | purified antibody |
| antibody product type | primary antibodies |
| clone【克隆】 | 5C6, monoclonal |
| form【形式】 | lyophilized |
| contains【包含】 | ≤0.1% sodium azide as preservative |
| species reactivity | human, mouse |
| manufacturer/tradename | Calbiochem® |
| storage condition【储存条件】 | OK to freeze |
| isotype【同位素/亚型】 | IgG1 |
| shipped in【运输】 | ambient |
| storage temp.【储存温度】 | −20℃ |
| packaging【包装】 | 100 μg in Plastic ampoule |
基本信息
| General description【一般描述】 | Recognizes the the ~50 kDa VASP protein phosphorylated at Ser157. This PhosphoDetect Mouse monoclonal antibody purified from serum-free cell culture supernatant by thiophilic adsorption and size exclusion chromatography. Recognizes the the ~50 kDa VASP protein phosphorylated at Ser157. |
| Immunogen【免疫原】 | Human a synthetic phosphopeptide corresponding to amino acids surrounding the Ser¹⁵⁷ phosphorylation site of human VASP |
| Application【应用】 | ELISA (0.05 µg/ml) Immunoblotting (1 µg/ml, colorimetric; 0.1 µg/ml, chemiluminescence) Immunocytochemistry (1-10 µg/ml) Immunoprecipitation (1-10 µg/10⁶ vanadate-treated A431 cells) |
| Warning【警告】 | Toxicity: Standard Handling (A) |
| Physical form【外形】 | Lyophilized from PBS, PEG, sucrose. |
| Reconstitution【重悬】 | Reconstitute in 1 ml sterile, dH₂O, 15 min, room temperature. Following reconstitution, refrigerate (4°C) for short-term storage or aliquot, flash-freeze in liquid nitrogen, and freeze (-70°C) for long-term storage. Stock solutions are stable for up to 3 months at 4°C or for up to 1 year at -70°C. Thaw aliquots at 37°C. Avoid freeze/thaw cycles of solutions. |
| Analysis Note【分析说明】 | Positive Control Vanadate-treated A431 cells |
| Other Notes【其他说明】 | Does not cross-react with non-phosphorylated VASP or with unrelated phosphorylation sites. For immunocytochemistry, fixation protocol may include up to 0.5% formaldehyde. Variables associated with assay conditions will dictate the proper working dilution. Bear, J.E., et al. 2001. Curr. Opin. Cell. Biol.13, 158. Lawrence, D.W., and Pryzwansky, K.B. 2001. J. Immunol.166, 5550. Burkhardt, M., et al. 2000. J. Biol. Chem.275, 33536. Harbeck, B., et al. 2000. J. Biol. Chem.275, 40. Smolenski, A., et al. 2000. J. Biol. Chem.275, 25723. |
| Legal Information【法律信息】 | CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany |
安全信息
| Storage Class Code【储存分类代码】 | 11 - Combustible Solids |
| WGK | WGK 1 |
