SEQPLEX-I WGA Kit

产品编号：

4151519

规格：	Whole Genome Amplification, DNA Amplification

包装规格：

24 REACTIONS，96 REACTIONS，384 REACTIONS

产品类别：

进口试剂

品牌：	Sigma-Aldrich

优惠价：

立即咨询

产品价格

产品编号	包装单位	单价(元)	国内现货	国外库存	询价单
4151519	24 REACTIONS	8800
4151519	96 REACTIONS	28000
4151519	384 REACTIONS	104250

产品别名

SEQPLEX-I WGA Kit

SeqX-i DNA Amplification kit

Whole Genome Amplification kit

WGA Kit

产品性质

technique(s)	DNA amplification: suitable PCR: suitable whole genome amplification: suitable
compatibility【相容性】	Illumina Next Generation Sequencing
shipped in【运输】	wet ice
storage temp.【储存温度】	−20℃

基本信息

General description【一般描述】	The SeqPlex-i WGA kit allows amplification of small quantities of DNA or from degraded/highly fragmented DNA for direct input onto Illumina^® next-generation sequencing (NGS) flow cells. The SeqPlex-i process is comprised of three steps: Pre-amplification/Library Synthesis, Amplification 1 and Amplification 2. Step 1: In the Pre-amplification/Library Synthesis step (using the Library Preparation Reagents), the template DNA is replicated using primers comprised of semi-degenerate 3′- ends with universal 5′- ends. As polymerization proceeds, displaced single strands serve as new templates for additional primer annealing and extension producing random, overlapping replicons flanked by a universal primer (5′) and primer complement (3′) sequence. Step 2: In the Amplified Library Synthesis step (using the Amplification 1 Reagents), products from pre-amplification/library synthesis are amplified by single primer PCR via the universal end sequence. These amplification products typically range from 200 to 500+ base pairs. Step 3: In the Sequencing Library Synthesis step (using Amplification 2 Reagents), single primer amplicons from amplification 1 are converted to dual Illumina^® primer PCR products ready for purification, quantification, and Illumina^® NGS.
Application【应用】	SeqX-i DNA Amplification Kit has been used for whole genome amplification.
Features and Benefits【特点和优势】	Amplifies fragmented/extremely small quantities of DNA: Yields from ChIP or FFPE, which are 200-500 bp are easily amplified by Random priming technology. Facilitates sequencing from as little as 100 pg of DNA Enhanced primers for complete genome coverage, minimal sequence bias, and amplicon size ideal for next generation sequencing (NGS) Cost-effective: No longer requires an additional NGS library prep step Compatible with Illumina^® next-generation sequencing
Other Notes【其他说明】	A 20 μL Amplification 2 reaction will produce >100 ng of amplified double-stranded cDNA when starting with 100 pg to 5 ng of high-quality DNA. Higher input quantities and higher quality DNA template generally result in increased yields. For damaged DNA, such as from FFPE, 1–50 ng input DNA is recommended. The dual index adapter primers (AP100) provided in this kit will only work for one sample. If pooling of samples for sequencing is required, the user must provide additional index primer sets. See example index primer sequences on page 2 of the technical bulletin.
Legal Information【法律信息】	Illumina is a registered trademark of Illumina, Inc.

产品说明

Disclaimer【免责声明】

The SeqPlex-I DNA Amplification Kit for whole genome amplification (WGA) is for R&D use only. Not for drug, household, or other uses.

安全信息

Storage Class Code【储存分类代码】	10 - Combustible liquids
WGK	WGK 3

关于我们	新闻中心	产品中心	订购指南	合作共赢
公司介绍联系方式在线咨询	伟伯动态行业资讯技术资料	进口试剂国产试剂标准品同位素添加剂香精香料仪器耗材	订购流程支付方式配送方式会员介绍	供应商合作经销商合作委托找货