Immobilon^®-FL PVDF Membrane

32031602

NB.22

The Immobilon^®-FL transfer membrane has a nominal pore size of 0.45 μm and is made up of polyvinylidene fluoride (PVDF) microporous membrane for binding proteins transferred from a variety of gel matrices. This membrane is hydrophobic and offers a uniformly controlled pore structure with a high binding capacity for biiomolecules. The membrane exhibits very low autofluorescence across a wide range of excitation/emission wavelengths. This property makes it ideal for blotting applications involving fluorescence-based immunodetection. In addition, the Immobilon^®-FL membrane can be used for standard chemiluminescent or chromogenic detection. The Immobilon^®-FL is recommended for optimal blotting of proteins with molecular weights greater than 20,000.

Immobilon^®-FL PVDF Membrane has been used in western blotting.

Immobilon^®-FL Membrane:

• Designed for optimal fluorescent immunodetection
• Extremely low background improves the sensitivity of all fluorescence detection protocols
• suitable for all commonly used fluorescent probes at all excitation and emission wavelengths
• compatible with standard blocking agents and buffers
• Ideal for multiplexing and chemifluorescence detection

Immobilon is a registered trademark of Merck KGaA, Darmstadt, Germany

PVDF membrane
plain filter
white filter

400

hydrophobic

Immobilon^®

western blot: suitable

20 cm × 20 cm

0.45 μm pore size

155 μg/cm² adsorption capacity (insulin)
205 μg/cm² adsorption capacity (BSA)
300 μg/cm² adsorption capacity (goat IgG)

for use with Amido black
for use with CPTS
for use with Coomassie brilliant blue
for use with Ponceau-S red

chemiluminescent
colorimetric
fluorometric

ambient

11 - Combustible Solids

WGK 3