Anti-CCRL2 (Mouse) Antibody, clone BZ2E3

32160702

NA.41

C-C chemokine receptor-like 2 (UniProt: O35457; also known as Chemokine receptor CCR11, G-protein coupled beta chemokine receptor, Lipopolysaccharide-inducible C-C chemokine receptor, L-CCR, CCRL2) is encoded by the Ccrl2 (also known as Ccr11, Lccr) gene (Gene ID: 54199) in murine species. CCRL2 is a seven transmembrane, G-protein coupled receptor that serves as a receptor for CCL19 and chemerin/RARRES2. It does not appear to be a signaling receptor, but may have a role in modulating chemokine-triggered immune responses by capturing and internalizing CCL19 or by presenting RARRES2 ligand to CMKLR1, a functional signaling receptor. CCRL2 is expressed by the majority of cell types of hematopoietic origin, including monocytes, macrophages, neutrophils, lymphocytes, and NK cells. In some cell types, its expression is upregulated by inflammatory stimuli. CCRL2 induction is shown to be dependent on NF- B and JAK/STAT signaling pathways. It is constitutively expressed by mast cells and is detected in bronchial epithelium in OVA-induced airway inflammation. It is reported to be up-regulated during dendritic cell (DC) maturation. It is also considered to be important for the development of Th2 responses and may act as a decoy receptor capturing and endocyting its ligands CCL5 and CCL19. (Ref.: Monnier, J. et al. (2012). J. Immunol. 189(2):956-967).

Clone BZ2E3 reacts with mCCRL2, but not mCMKLR1, huCMKLR1, mCRTH2, huCCRL2, or mCCR10. Clone BZ2E3 immunostained the surface of mCCRL2-expressing cells, but not non-mCCRL2-expressing cells (Monnier, J., et al. (2012). J. Immunol. 189(2):956-967; Zabel, B.A., et al. (2008). J. Exp. Med. 205(10):2207-2220).

KLH-conjugated linear peptide corresponding to the N-terminal end sequence of mouse CCRL2 (Zabel, B.A., et al. (2008). J. Exp. Med. 205(10):2207-2220).

Flow Cytometry Analysis: 10-100 µg/mL from a representative lot immunostained mouse peritoneal exudate cells from wild-type, but not Ccrl2-/- mice (Courtesy of Brian Zabel, Ph.D., Stanford University, U.S.A.).

Flow Cytometry Analysis: A representative lot immunostained liver and lung CD31+/CD146+ endothelial cells from wild-type, but not Ccrl2-/- mice. Intraperitoneal LPS injection prior to cells isolation upregulated surface CCRL2 staining on liver and lung CD31+/CD146+ endothelial cells (Monnier, J., et al. (2012). J. Immunol. 189(2):956-967).

Flow Cytometry Analysis: A representative lot detected cytokines-induced CCRL2 expression on the surface of bEND.3 mouse endothelioma cells. IKKbeta inhibitor BAY (11 7082) and/or JAK-1 inhibitor (sc-204021) inhibited cytokines-induced CCRL2 expression (Monnier, J., et al. (2012). J. Immunol. 189(2):956-967).

Flow Cytometry Analysis: A representative lot immunostained L1.2 mouse pre-B lymphoma cells expressing transfected mCCRL2, but not untransfected L1.2 or L1.2 transfectants expressing mCMKLR1, huCMKLR1, mCRTH2, huCCRL2, or mCCR10, nor non-CCRL2-expressing mouse blood cells. Chemerin, but not CCL2, blocked clone BZ2E3 staining of CCRL2-positive mouse peritoneal exudate cells (Zabel, B.A., et al. (2008). J. Exp. Med. 205(10):2207-2220).

This rat monoclonal Anti-CCRL2 (Mouse) Antibody, clone BZ2E3, Cat. No. MABF1033, is validated for use in Flow Cytometry for the detection of CCRL2.
Research Category
Inflammation & Immunology

Identity Confirmation by Isotyping Test.

Isotyping Analysis: The identity of this monoclonal antibody is confirmed by isotyping test to be rat IgG2a .

Protein G purified.
Format: Purified
Purified rat IgG2a in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Concentration: Please refer to lot specific datasheet.

100

rat

purified antibody

primary antibodies

BZ2E3, monoclonal

mouse

human

flow cytometry: suitable

IgG2aκ

NP_001289305.1

O35457

ambient

40.75 kDa calculated.

Stable for 1 year at 2-8°C from date of receipt.

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

12 - Non Combustible Liquids

WGK 1