Anti-Cdc4 Antibody

32160702

NA.41

The F-box protein family is characterized by an approximately 40 amino acid motif, the F-box. F-box proteins are divided into 3 classes: FBWs containing WD-40 domains, FBLs containing leucine-rich repeats, and FBXs containing either different protein-protein interaction modules or no recognizable motifs. They constitute one of the four subunits of ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination.
Cdc4 is one member of this family, which is also known as AGO, FBW7, FBX30 or SEL10. CDC4 binds directly to cyclin E and targets cyclin E for ubiquitin-mediated degradation. Cdc4 proteins of amino acid lengths varying from 553 aa to 707 aa in length have been reported. Cdc4 is an inhibitor of Notch signaling that targets Notch for ubiquitin-mediated degradation after a nuclear phosphorylation event.5,6 Cdc4 interacts with presenilin 1, facilitates its ubiquitination, and alters A-beta peptide production. Thus, it may be important for Alzheimer′s disease. Mutations of the CDC4 gene are detected in ovarian and breast cancer cell lines and the CDC4 gene may also be involved in the pathogenesis of human pancreatic and endometrial cancers.

Recognizes CDC4.
Reactivity has been confirmed with human T47D, U2-OS, MCF-7, HepG2 and HeLa cell lysates.

Synthetic peptide derived from an internal region of the human and mouse Cdc4 (archipelago (AGO), F-box/WD-repeat protein 7, F-box and WD-40 domain protein 7, F-box protein FBW7, FBX30 and SEL-10).

Western Blotting: 1-3 μg/mL
Immunoprecipitation: 7 µg/reaction
Optimal working dilutions must be determined by end user.
Anti-Cdc4 Antibody is an antibody against Cdc4 for use in WB & IP.
Research Sub Category
Ubiquitin & Ubiquitin Metabolism
Research Category
Protein Trafficking

ImmunoAffinity Purified
This polyclonal antibody is supplied in 400 μL of phosphate buffered saline (pH 7.4) containing 0.1% sodium azide.

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

rabbit

100

affinity isolated antibody

primary antibodies

polyclonal

affinity chromatography

human

Chemicon^®

immunoprecipitation (IP): suitable
western blot: suitable

NM_001013415.1
NM_018315.4
NM_033632.2

Q969H0

dry ice

human ... FBXW7(55294)

Store at -20°C in undiluted aliquots for 1 year from date of shipment.

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

12 - Non Combustible Liquids

WGK 2