Anti-CD44v10 Antibody, exon v10

32160702

NA.41

CD44v10 Approximant 116 kDa)

Recent studies indicate that the expression of CD44 on the cell surface changes profoundly during tumor metastasis, particularly during the progression of various carcinomas. Sequencing analysis reveals that CD44 exists in different forms (at least 15 isoforms have been identified) that are variants of the common or standard hemopoietic form CD44s. Specifically, the human CD44 gene contains 19 exons, out of which 10 exons can potentially be alternatively spliced. In particular, CD44s contains exons 1-15 and exons 15-19. The other exons (e.g. 6-14) are also spliced in a variety of different cells. For example, epithelial cells contain exons 12-14 inserted into the CD44s transcripts. This isoform has been designated as CD44E. Other CD44 variants CD44v) have the same sequences at the two ends of the molecule, but differ in a middle region (with different numbers of additional exons) located on the external side of the cell membrane. It has been suggested that expression of CD44 variants with the additional exon 14 (so-called v10) is responsible for tumor progression and metastasis. In fact, CD44v10 expression has been used as an indicator of metastasis.

Epitope: exon v10
Synthetic peptide of human CD44v10

This Anti-CD44v10 Antibody, exon v10 is validated for use in ELISA, FC, IP, RIA, WB, IC, IH(P) for the detection of CD44v10.
ELISA: 1:2000
Flow Cytometry: 1:1000
Immunofluorescence: 1:200
Immunohistostaining of paraffin embedded tissue: 1:250
Immunoprecipitation: 1:200
Western Blotting: 1:1000
RIA: 1:5000

Optimal working dilutions must be determined by the end user.

Replaces: 04-1123

Format: Purified
Purified immunoglobulin. Liquid in 10 mM sodium phosphate, 150 mM NaCl with 0.01% sodium azide.

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

rabbit

100

purified immunoglobulin

primary antibodies

polyclonal

rat, rabbit, mouse, pig, bovine, human, canine

Chemicon^®

ELISA: suitable
flow cytometry: suitable
immunocytochemistry: suitable
immunohistochemistry: suitable (paraffin)
immunoprecipitation (IP): suitable
radioimmunoassay: suitable
western blot: suitable

NM_000610.3
NM_001001389.1
NM_001001390.1
NM_001001391.1
NM_001001392.1

P16070

dry ice

human ... CD44(960)

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

10 - Combustible liquids

WGK 2