Anti-Diglycyl Lysine (Clone GX41) Antibody

32160702

NA.41

Anti-Diglycyl Lysine has been shown to specifically detect peptides derived from the ubiquitinated portion of proteins. Diglycyl Lysine, clone GX41 is thought to find diglycyl-lysine moieties that are created and left behind at places of ubiquitination after digestion with trypsin has occurred.

Antibody recognizes lysine residues modified by diglycine, a residue left at sites of ubiquitination after trypsin digestions.
Broad species cross-reactivity expected based on 100% sequence homology.

Linear peptide corresponding to Diglycyl Lysine.

Research Sub Category
General Post-translation Modification
Research Category
Signaling
Immunoprecipitation Analysis: 10 µg of Anti-Diglycyl Lysine immunoprecipitated peptides containing Diglycyl Lysine modification from isolated ubiquitinated proteins which were obtained from a human cell line.

Mass Spectrometry Analysis: A previous lot was used by an independent a laboratory in Mass Spec. Image courtesy of Samie Jaffrey, Cornell Medical College.
Anti-Diglycyl Lysine (Clone GX41) Antibody detects level of Diglycyl Lysine (Clone GX41) & has been published & validated for use in WB & IP.

Evaluated by Western Blot in BSA protein and Diglycine modified BSA.

Western Blot Analysis: 0.5 µg/mL of this antibody detected Diglycyl Lysine on 10 µg of BSA protein and Diglycine modified BSA.

Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Format: Purified
Protein G

Control
BSA protein and Diglycine modified BSA

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

100

mouse

purified immunoglobulin

primary antibodies

GX41, monoclonal

rat, human, mouse

all

immunoprecipitation (IP): suitable
western blot: suitable

IgG1κ

wet ice

Molecular weight can vary depending on the size of the protein or peptides containing Diglycyl modified lysine residues.

Stable for 1 year at 2-8°C from date of receipt.

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

12 - Non Combustible Liquids

WGK 1