Anti-Dishevelled-1 Antibody

32160702

NA.41

DVL1, the human homolog of the Drosophila dishevelled gene (dsh) encodes a cytoplasmic phosphoprotein that regulates cell proliferation. The protein acts as a transducer molecule for developmental processes, including segmentation and neuroblast specification. DVL1 mutation is likely responsible for transformation in neuroblastomas, and the Schwartz-Jampel syndrome and Charcot-Marie-Tooth disease type 2A have been mapped to the same region as DVL1 gene, thus DVL1 maybe important in these diseases as well.

Dishevelled-1 protein (dvl-1). By Western blot the antibody shows no cross reactivity with recombinant dishevelled -2 or dishevelled -3 protein Pre-adsorption of the antibody with the immunogen peptide completely abolishes its reactivity by Western blot against dishevelled -1 protein.

Synthetic peptide corresponding to amino acids 557-571 of human dishevelled-1 protein.

Research Category
Stem Cell Research
Anti-Dishevelled-1 Antibody is an antibody against Dishevelled-1 for use in WB.
Western blot: 1:500 using ECL. The antibody reacts with a protein of ~85kDa. Dilute antibody in 50 mM PBS, pH 7.4 containing 1.5% sodium chloride and 1% normal animal serum (same species as the host of the secondary antibody).

Optimal working dilutions must be determined by end user.
Research Sub Category
Hematopoietic Stem Cells

Neural Stem Cells

Liquid in PBS, pH 7.4, containing 0.01M sodium azide.

Control
SK-N-SH (neuroblastoma) whole cell lysates

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

rabbit

100

affinity purified immunoglobulin

primary antibodies

polyclonal

affinity chromatography

human

Chemicon^®

western blot: suitable

sample type neural stem cell(s)
sample type hematopoietic stem cell(s)

NM_004421.2
NM_181870.1
NM_182779.3

O14640

wet ice

85 kDa

Maintain at -20°C in undiluted aliquots (not less than 10 μL) for up to 6 months. Avoid repeated freeze/thaw cycles. The use of high quality antiserum-grade plastic or glass is recommended.

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

10 - Combustible liquids

WGK 2