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Anti-GATA4 Antibody

产品编号:4154413
规格:Chemicon®, from rabbit
包装规格:100 μG
产品类别:进口试剂
品牌:Sigma-Aldrich
优惠价:立即咨询
产品价格
产品编号包装单位单价(元)国内现货国外库存询价单
4154413100 μG5420
基本信息
eCl@ss
32160702
NACRES
NA.41
General description【一般描述】
GATA4 (GATA binding factor-4) is a member of the GATA family of transcription factors. GATA4 is involved in embryogenesis and in myocardial differentiation and function and is associated with heart septal defects.
Specificity【特异性】
Recognizes GATA4.
Immunogen【免疫原】
Synthetic peptide corresponding to amino acids 313-331 of human GATA4 (RKRKPKNLNKSKTP).
Application【应用】
Research Sub Category
Transcription Factors
Western Blot and Immunocytochemistry: 1/200 to 1/1000.

Optimal dilutions must be determined by the user.
Research Category
Epigenetics & Nuclear Function
Detect GATA4 with Anti-GATA4 Antibody (Rabbit Polyclonal Antibody), that has been shown to work in ICC & WB.
Linkage【联系】
Replaces: MABE477
Physical form【外形】
Affinity Purified immunoglobulin precipitated in a solution of 50% saturated ammonium sulfate and PBS containing no preservatives.
ImmunoAffinity Purified
Analysis Note【分析说明】
Control
Heart tissue
Legal Information【法律信息】
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
产品性质
biological source【生物来源】
rabbit
Quality Level【质量水平】
100
antibody form【抗体形式】
affinity purified immunoglobulin
antibody product type
primary antibodies
clone【克隆】
polyclonal
purified by【纯化方式】
affinity chromatography
species reactivity
rat, human, mouse
manufacturer/tradename
Chemicon®
technique(s)
immunocytochemistry: suitable
western blot: suitable
NCBI accession no.【NCBI登记号】
NM_002052.2
UniProt accession no.【UniProt登记号】
P43694
shipped in【运输】
wet ice
产品说明
Target description【目标描述】
45 kDa
Storage and Stability【储存及稳定性】
Maintain unopened vial at -20°C for up to 6 months. Avoid repeated freeze/thaw cycles.

The rehydrated antibody solutions can be stored undiluted at 2–8°C for 2 months without any significant loss of activity. Note, the solution is not sterile, thus care should be taken if product is stored at 2–8°C.

For storage at -20°C, the addition of an equal volume of glycerol can be used, however, it is recommended that ACS grade or higher glycerol be used, as significant loss of activity can occur if the glycerol used is not of high quality.

For freezing, it is recommended that the rehydrated antibody solution be further diluted 1:1 with a 2% BSA (fraction V, highest-grade available) solution made with the rehydration buffer. The resulting 1% BSA/antibody solution can be aliquoted and stored frozen at -70°C for up to 6 months. Avoid repeated freeze/thaw cycles.

PREPARATION AND USE:

To reconstitute the antibody, centrifuge the antibody vial at moderate speed (5,000 rpm) for 5 minutes to pellet the precipitated antibody product. Carefully remove the ammonium sulfate/PBS buffer solution and discard. It is not necessary to remove all of the ammonium sulfate/PBS solution: 10 μL of residual ammonium sulfate solution will not effect the resuspension of the antibody. Do not let the protein pellet dry, as severe loss of antibody reactivity can occur.

Resuspend the antibody pellet in any suitable biological buffer, standard PBS or TBS (pH 7.3–7.5) are typical. Volumes required are not critical but it is suggested that the final antibody concentration be between 0.1 mg/mL and 1.0 mg/mL. For example, to achieve a1 mg/mL concentration with 50 μg of precipitated antibody, the amount of buffer needed would be 50 μL.

Carefully add the liquid buffer to the pellet. DO NOT VORTEX. Mix by gentle stirring with a wide pipet tip or gentle finger-tapping. Let the precipitated antibody rehydrate for 1 hour at 4–25°C prior to use. Small particles of precipitated antibody that fail to resuspend are normal. Vials are overfilled to compensate for any losses.
Disclaimer【免责声明】
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
安全信息
Storage Class Code【储存分类代码】
12 - Non Combustible Liquids
WGK
WGK 2
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