Anti-Guanylate Cyclase 2E Antibody, clone 8A5

32160702

Guanylyl cyclase GC-E (UniProt: P52785; also known as EC: 4.6.1.2, Guanylate cyclase 2E, GC-2E) is encoded by the Gucy2e (also known as Guc2e) gene (Gene ID: 14919) in murine species. GC-2E is a single-pass type I membrane protein that plays a specific functional role in the rods and/or cones of photoreceptors. It may be the enzyme involved in the re-synthesis of cGMP required for recovery of the dark state after phototransduction. Following photoexcitation, cGMP in photoreceptors is first hydrolyzed by a light-activated phosphodiesterase (PDE6) and then replenished by a retinal membrane guanylyl cyclase. It is synthesized with a 50 amino acid signal peptide that is cleaved to generate the mature enzyme. It consists of an extracellular domain (aa 51-469), a helical domain (aa 470-490) and a cytoplasmic domain (aa 491-1108).

Clone 8A5 detects Guanylate cyclase-1 in Bovine, Human, and murine species. It targets an epitope within 48 amino acids from the C-terminal end.

GST-tagged recombinant fragment corresponding to 48 amino acids from the C-terminal end of human Guanylate cyclase-1.

Epitope: C-terminus

Anti-Guanylate Cyclase-2E, clone 8A5, Cat. No. MABN2436, is a highly specific mouse monoclonal antibody that targets retinal Guanylyl cyclase (RETGC-1; GC-2E) and has been used in Immunofluorescence and Western Blotting.
Research Category
Neuroscience
Immunofluorescence Analysis: A representative lot detected Guanylate Cyclase-1 in mouse retina tissues (Courtesy of Dr. Robert Molday, Ph.D., University of British Columbia, Canada).

Immunofluorescence Analysis: A representative lot detected Guanylate Cyclase-1 in Immunofluorescence applications (Molday, L.L., et. al. (2013). Hum Mol Genet. 22(19):3894-905).

Western Blotting Analysis: 10 ug/mL of a representative lot detected Guanylate Cyclase-1 in mouse retina tissue lysates (Courtesy of Dr. Robert Molday, Ph.D., University of British Columbia, Canada).

Western Blotting Analysis: A representative lot detected Guanylate Cyclase-1 in Western Blotting applications (Peshenko, I.V., et. al. (2011). Biochemistry. 50(44):9511-9; Molday, L.L., et. al. (2013). Hum Mol Genet. 22(19):3894-905)

Isotype testing: Identity Confirmation by Isotyping Test.

Isotyping Analysis: The identity of this monoclonal antibody is confirmed by isotyping test to be mouse IgG1 .

Protein G purified
Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Format: Purified

Concentration: Please refer to lot specific datasheet.

100

mouse

purified immunoglobulin

primary antibodies

8A5, monoclonal

human, mouse, bovine

antibody small pack of 25 μg

immunofluorescence: suitable
western blot: suitable

IgG1κ

NP_032218

P52785

ambient

~120 kDa observed; 120.06 kDa calculated. Uncharacterized bands may be observed in some lysate(s).

Stable for 1 year at 2-8°C from date of receipt.

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

12 - Non Combustible Liquids

WGK 1