Anti-H2AK15ub Antibody, clone EDL H2AK15-4

32160702

Histone H2A is one of four components of the core nucleosomal structure. Histones undergo a number of post-translational modifications (PTM) in response to various stimuli that may induce changes in the structure of the nucleosome and hide or expose DNA sequences. The degree of ubiquitylation at DNA damage sites is regulated at multiple levels in order to generate proper DNA damage response. Uncontrolled ubiquitylation of histones can lead to unscheduled transcriptional silencing and generate defects in DNA damage checkpoint activation and cell cycle arrest. The RNF168 E3 ligase ubiquitylates H2A at K13, K15, which in turn triggers the recruitment of DNA damage response proteins, such as 53BP1 and BRCA1 to DNA break sites. USP51, a deubiquitinating enzyme, removes H2AK13, 15ub following DNA repair. Depletion of USP51 can result in elevated levels of H2AK13, 15ub on chromatin and cause delays in disassembly of proteins at DNA damage foci.

Clone EDL H2AK15-4 specifically recognizes histone H2A monoubiquitylated at Lys15, but not unmodified H2A. Does not recognize di-ubiquitin, K63 and K27 linked di-ubiquitin species.

KLH-conjugated branched peptide corresponding to the ubiquitination site on human H2AK15.

Anti-H2AK15ub, clone EDL H2AK15-4, Cat. No. MABE1119, is a highly specific mouse monoclonal antibody, that targets H2A ubiquitylated at Lys15 and has been tested in Immunocytochemistry and Western Blotting.
Western Blotting Analysis: A representative lot detected H2AK15ub in U2OS cells with or without RNF168 depletion (Wang, Z., et. al. (2016). Genes Dev. 30(8):946-59).


Immunocytochemistry Analysis: A representative lot detected H2AK15ub in U2OS cells (Wang, Z., et. al. (2016). Genes Dev. 30(8):946-59).

Evaluated by Western Blotting in Ub-Lysine15 H2A and H2AK13, 15R nucleosome lysates.


Western Blotting Analysis: 2 ug/mL of this antibody detected Ub-Lysine15 H2A and H2AK13, 15R nucleosome lysates.

2 mg/mL

Protein G purified
Format: Purified

Concentration: Please refer to lot specific datasheet.

100

mouse

purified immunoglobulin

primary antibodies

EDL H2AK15-4, monoclonal

mouse, human, Drosophila

immunocytochemistry: suitable
western blot: suitable

IgG2bκ

NP_003500.1

P0C0S8

ambient

human ... HIST1H2AG(8969)

~25 kDa observed. Uncharacterized bands may be observed in some lysate(s).

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