Anti-HCN1 Antibody, clone N70/28

32160702

NA.41

HCN1 is a member of a family of pacemaker channels activated by hyperpolarization and regulated by cyclic nucleotides. HCN1 and HCN2 proteins play an important role for motor learning and synapse formation through integration of cerebellar Purkinje cells. The two proteins also shape autonomous activity of single neurons and the periodicity of network oscillations. HCN1 expression is particularly high in the cerebral cortex, hippocampus, cerebellum, and facial motor nucleus. Hyperpolarization of activated cation channels of the HCN gene family such as HCN1, contributes to spontaneous rhythmic activity in both heart and brain. HCN channels pass both Na+ and K+ with relative permeability ratio of ~0.15 to 0.25. The primary sequence of HCN pore region is clearly related to that of highly selective K+ channels. In contrast to nonselective CNG or Na+-channels, both K+ and HCN channels contain a GYG motif in the pore.

This antibody recognizes HCN1 at the cytoplasmic domain.

Recombinant protein corresponding to rat HCN1 at the cytoplasmic domain.
Epitope: Cytoplasmic domain

Immunohistochemistry Analysis: 1:300 dilution from a previous lot detected HCN1 in rat cerebellum and rat brain/inferior colliculus tissue.
Detect HCN1 using this Anti-HCN1 Antibody, clone N70/28 validated for use in WB, IH.
Research Category
Neuroscience
Research Sub Category
Ion Channels & Transporters

Evaluated by Western Blot in rat brain lysate.

Western Blot Analysis: 2 µg/mL of this antibody detected HCN1 on 10 µg of rat brain lysate.

Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide.
Format: Purified
Protein G

Control
Rat brain lysate

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

100

mouse

purified immunoglobulin

primary antibodies

N70/28, monoclonal

rat

immunohistochemistry: suitable
western blot: suitable

IgG1κ

NP_445827

Q9JKB0

wet ice

human ... HCN1(348980)

~120 kDa observed; consistent with a glycosylation of a 100kDa protein.

Stable for 1 year at 2-8°C from date of receipt.

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

12 - Non Combustible Liquids

WGK 1