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Anti-Histone H3, Acetylated (1-20) Rabbit pAb
产品性质
| biological source【生物来源】 | rabbit |
| Quality Level【质量水平】 | 100 |
| antibody form【抗体形式】 | purified antibody |
| antibody product type | primary antibodies |
| clone【克隆】 | polyclonal |
| form【形式】 | liquid |
| contains【包含】 | ≤0.1% sodium azide as preservative |
| species reactivity | Tetrahymena sp., human |
| manufacturer/tradename | Calbiochem® |
| storage condition【储存条件】 | OK to freeze avoid repeated freeze/thaw cycles |
| isotype【同位素/亚型】 | IgG |
| shipped in【运输】 | wet ice |
| storage temp.【储存温度】 | −20℃ |
| packaging【包装】 | 50 μg in Plastic ampoule Please refer to vial label for lot-specific concentration. |
基本信息
| General description【一般描述】 | Anti-Histone H3, Acetylated (1-20), rabbit polyclonal, recognizes the ~17 kDa acetylated histone H3 protein. It is validated for use in Western blotting, immunocytochemistry & immunoprecipitation. Recognizes the ~17 kDa acetylated histone H3 protein. Protein A purified rabbit polyclonal antibody. Recognizes the ~17 kDa acetylated histone H3 protein. |
| Immunogen【免疫原】 | Human a diacetylated synthetic peptide [ARTKQTARAcKSTGGAcKAPRKQL(C)] corresponding to amino acids 1-20 of human histone H3, conjugated to KLH |
| Application【应用】 | Immunoblotting (10-500 ng/ml, chemiluminescence) Immunocytochemistry (5 µg/ml) Immunoprecipitation (see comments) |
| Warning【警告】 | Toxicity: Standard Handling (A) |
| Physical form【外形】 | In 150 mM NaCl, 100 mM Tris-glycine, pH 7.4. |
| Reconstitution【重悬】 | Following initial thaw, aliquot and freeze (-20°C). |
| Analysis Note【分析说明】 | Positive Control HeLa cells |
| Other Notes【其他说明】 | Braunstein, M., et al. 1996. Mol. Cell. Biol.16, 4349. Braunstein, M., et al. 1993. Genes Devel.7, 592. Perry, C.A., et al. 1993. Biochemistry32, 13605. Lin, R., et al. 1989. J. Cell. Biol.108, 1577. For immunocytochemistry, HeLa cells were fixed with 95% ethanol/5% acetic acid and treated with 5 mM sodium butyrate, an inhibitor of histone deacetylases, for 24 h. 10-500 ng/ml of antibody detected acetylated histone H3 in acid-extracted proteins from HeLa cells. Using sodium butyrate, an inhibitor of histone deacetylases, enhances detection of acetylated histone H3. Higher concentrations of antibody also detected unacetylated histone H3. Preliminary evidence from an independent library suggests that phosphorylation of Ser10 on histone H3 may disrupt epitope recognition by this antibody. This antibody has also been reported to work for immunoprecipitation. Variables associated with assay conditions will dictate the optimal working dilution. |
| Legal Information【法律信息】 | CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany |
安全信息
| Storage Class Code【储存分类代码】 | 10 - Combustible liquids |
| WGK | WGK 1 |
