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Anti-Hsp70 Mouse mAb (C92F3A-5)
产品别名
Anti-Hsp70 Mouse mAb (C92F3A-5)
Anti-Heat Shock Protein 70
产品性质
| Quality Level【质量水平】 | 100 |
| biological source【生物来源】 | mouse |
| antibody form【抗体形式】 | purified antibody |
| antibody product type | primary antibodies |
| clone【克隆】 | C92F3A-5, monoclonal |
| form【形式】 | liquid |
| contains【包含】 | ≤0.1% sodium azide as preservative |
| species reactivity (predicted by homology) | all |
| manufacturer/tradename | Calbiochem® |
| storage condition【储存条件】 | OK to freeze avoid repeated freeze/thaw cycles |
| isotype【同位素/亚型】 | IgG1 |
| shipped in【运输】 | wet ice |
| storage temp.【储存温度】 | −20℃ |
| packaging【包装】 | 50 μg in Plastic ampoule Please refer to vial label for lot-specific concentration. |
基本信息
| General description【一般描述】 | Mouse monoclonal antibody purified by ion-exchange chromatography. Recognizes the ~70 kDa Hsp70 protein. Anti-Hsp70, mouse monoclonal, clone C92F3A-5, recognizes the ~70 kDa Hsp70. Does not cross-react with Hsc70. It is validated for use in ELISA, FC, WB, ICC, IP & IHC (frozen and paraffin sections). Recognizes the ~70 kDa Hsp70 protein. Does not cross-react with Hsc70. |
| Immunogen【免疫原】 | Hsp70 from HeLa cells Human |
| Application【应用】 | ELISA (see comments) Flow Cytometry (see comments) Frozen Sections (5 µg/ml) Immunoblotting (1 µg/ml) Immunocytochemistry (5 µg/ml) Immunoprecipitation (see comments) Paraffin Sections (5 µg/ml) |
| Warning【警告】 | Toxicity: Standard Handling (A) |
| Physical form【外形】 | In PBS, 50% glycerol, pH 7.2. |
| Reconstitution【重悬】 | Following iniital thaw, aliquot and freeze (-20°C). |
| Analysis Note【分析说明】 | Positive Control L929 cells, Human colon cancer tissue |
| Other Notes【其他说明】 | Does not cross-react with Hsc70. Because Hsp70 is not expressed constitutively in most cells, this antibody is suitable for ascertaining whether a stress-response has occurred in the cell. Increased levels of Hsp70 expression occurs following stress even in cells that constitutively express Hsp70. This antibody has also been reported to work for ELISA, flow cytometry, and immunoprecipitation. Variables associated with assay conditions will dictate the optimal working dilution. Hang, H., and Fox, M.H. 1995. Cytometry19, 119. Kilgore, J.L., et al. 1994. J. Appl. Physiol.76, 589. Heufelder, A.E., et al. 1992. J. Clin. Endocrinol. Metab.74, 724. Gower, D.J., et al. 1989. J. Neurosurg.70, 605. Milarski, K., et al. 1989.J. Cell Biol.108, 413. Vass, K., et al. 1988. Acta Neuropathologica77, 128. Welch, W.J., and Suhan, J.P. 1986. J. Cell Biol.103, 2035. |
| Legal Information【法律信息】 | CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany |
安全信息
| Storage Class Code【储存分类代码】 | 10 - Combustible liquids |
| WGK | WGK 1 |
