Anti-Hsp47 Mouse mAb (M16.10A1)

100

mouse

purified antibody

primary antibodies

M16.10A1, monoclonal

liquid

≤0.1% sodium azide as preservative

bovine, monkey, mouse, human, guinea pig, canine, rat, sheep, porcine, chicken, hamster

Calbiochem^®

OK to freeze
avoid repeated freeze/thaw cycles

IgG2b

wet ice

−20℃

50 μg in Plastic ampoule
Please refer to vial label for lot-specific concentration.

Protein G purified mouse monoclonal antibody. Recognizes the ~47 kDa Hsp47 protein.
Recognizes the ~47 kDa Hsp47 protein.
Anti-Hsp47, mouse monoclonal, clone M16.10A1, recognizes the ~47 kDa Hsp47 protein in wide variety of species. It is validated for use in ELISA, EIA, WB, ICC, IF, IP, and IHC (paraffin sections).

Rat
Hsp47 from L6 cells

ELISA (see comments)

Enzyme Immunoassay (see comments)

Immunoblotting (1 µg/ml, colorimetric)

Immunocytochemistry (1 µg/ml)

Immunofluorescence (5 µg/ml)

Immunoprecipitation (5 µg/ml)

Paraffin Sections (see comments)

Toxicity: Standard Handling (A)

In PBS, 50% glycerol, pH 7.2.

Following initial thaw, aliquot and freeze (-20°C).

Positive Control
3T3 cells, Human breast cancer tissue

Kuroda, K., et al. 1998. J. Invest. Dermatol.111, 1023.
Nandan, D., et al. 1998. Exp. Cell Res.179, 289.
Clark, E.P., et al. 1991. J. Biol. Chem.266, 17230.
Vaillancourt, J.P., and Cates, G.A. 1991. Biochem. J.274, 793.
Cates, G.A., et al. 1987. Biochem. Cell Biol. 65, 767.
Saga, S., et al. 1987. J. Cell Biol.105, 517.
Nagata, K., et al. 1986. J. Biol. Chem. 261, 7531.
This antibody has also been reported to work for ELISA and enzyme immunoassay. Variables associated with assay conditions will dictate the optimal dilution.

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10 - Combustible liquids

WGK 1