Anti-Integrin α2 Antibody

32160702

NA.41

Human alpha2 integrin subunit. Specificity determined by immunoprecipitation from a detergent extract of 35S methionine labeled endothelial cells. The epitope recognized is on the cytoplasmic domain of the protein and is thus intracellular. Shows no cross-reactivity to alpha1, alpha3, alpha4, alpha5, alpha6, or alphaV subunits.

Synthetic peptide derived from the COOH terminal sequence (cytoplasmic domain) of the human alpha2 integrin subunit {KYEKMTKNPDEODETTELSS}

Research Category
Cell Structure
Anti-Integrin α2 Antibody detects level of Integrin α2 & has been published & validated for use in ELISA, IF, IH, IP, RIA & WB.
Research Sub Category
Integrins
Immunofluorescence: 1:500 (suggested for use on acetone-fixed tissue only)

ELISA/RIA: 1:1000

Western blot: 1:1000 The antibody has been used to report alpha2 expression in HUVECs during tubule-like structure formation in collagen (Deroanne, 1996). Recognizes ~160kDa alpha 2 subunit

Immunoprecipitation: 5 μL precipitates alpha2/beta1 from 5 x 106 cells.

Immunohistochemistry: 1:500

Optimal working dilutions must be determined by end user.

Neat rabbit antiSerum. Liquid with 0.05% sodium azide
Unpurified

Control
Skin (basement membrane), High grade ovarian tumors

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

rabbit

100

serum

primary antibodies

polyclonal

mouse, chicken, hamster, rat, human

Chemicon^®

ELISA: suitable
immunofluorescence: suitable
immunohistochemistry: suitable
immunoprecipitation (IP): suitable
radioimmunoassay: suitable
western blot: suitable

NM_002203.3

P17301

wet ice

human ... ITGA2(3673)

160kDa

Maintain for 1 year at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

10 - Combustible liquids

WGK 1