Anti-IP3 Receptor 2 Antibody

32160702

NA.41

IP3 Receptor 2.

Synthetic peptide from the C terminus of human IP3 Receptor 2.

Research Category
Signaling
Detect IP3 Receptor 2 using this Anti-IP3 Receptor 2 Antibody validated for use in IC, IH & WB.
Western blot: 1:1,000 using ECL. The antibody reacts with the 250 kDa IP3 Receptor 2 protein in microsomal preparations. Suggested blocking buffer is 10% normal goat serum (or same host as your secondary antibody), 1% BSA in 0.1M PBS with 0.05% Tween 20. Suggested dilution buffer is 1% normal goat serum (or same host as your secondary antibody), 1% BSA in 0.1M PBS with 0.05% Tween 20. Preferred gel percentage is 4-12% gradient gel or 5% PAGE. For microsomal methods and blotting procedure similar antibodies can be found in: Perez, et al. J Biol. Chem. 272:23961

http://www.jbc.org/cgi/reprint/272/38/23961?ijkey=35bcf508a3025fc8bf8203ea3805122eec0caf1e )

Immunocytochemistry: 1:1,000

Immunohistochemistry: 1:1,000 overnight at 2-8°C using a fluorescently labeled secondary antibody. Suggested fixative is 4% paraformaldehyde in 0.1M PBS (one hour). Suggested permeablization method is 0.05% Triton X-100 in dilution buffer. Suggested blocking buffer is 10% normal goat serum (or same host as your secondary antibody) and 1% BSA in 0.1M PBS. Suggested dilution buffer is 1% normal goat serum (or same host as your secondary antibody) and 1% BSA in 0.1M PBS.

Optimal working dilutions must be determined by the end user.
Research Sub Category
Lipid Signaling

Rabbit serum. Liquid.

Control
Western blot = adult mouse liver.

IHC = adult mouse hepatocytes (adult mouse cerebellum Purkinje cells will be negative).

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

rabbit

100

serum

primary antibodies

polyclonal

human, mouse

Chemicon^®

immunocytochemistry: suitable
immunohistochemistry: suitable
western blot: suitable

NM_002223.2

Q14571

wet ice

human ... ITPR2(3709)

Maintain at -20°C in undiluted aliquots for up to 6 months after date of receipt. Avoid repeated freeze/thaw cycles.

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

10 - Combustible liquids

WGK 1