Anti-LAMP-3 Antibody, clone 16H11.2

32160702

NA.41

Lysosyme-associated membrane proteins (LAMP) are transmembrane lysosomal glycoproteins, which share a common Gly-Tyr motif as a lysosomal targeting signal. While LAMP-1 and LAMP-2 contain type 1 transmembrane domains, LAMP-3 contains a transmembrane 4 superfamily domain, tetraspanin. FceRI-mediated stimulation of basophils causes LAMP-3 to be translocated from its intracellular localization to the plasma membrane. LAMP-3 thereby serves as a reliable marker for basophil activation. LAMP-3 expression is known to be reported in human bone marrow mast cells and in leukemic human mast cell line (HMC-1), among other cell lines. LAMP3 is shown to be regulated by hypoxia in a panel of tumor cells, via the unfolded protein response (UPR). UPR is a mechanism of adaptation to endoplasmic reticulum stress and is demonstrated to contribute to hypoxic adaptation in tumors through multiple mechanisms.

Recombinant protein corresponding to human LAMP-3.

Research Category
Cell Structure
Immunohistochemistry Analysis: A 1:200 dilution from a representative lot detected LAMP-3 in colorectal adenocarcinoma tissue.
Research Sub Category
Apoptosis - Additional

Organelle & Cell Markers
Detect LAMP-3 using this Anti-LAMP-3 Antibody, clone 16H11.2 validated for use in WB, IH.

Evaluated by Western Blot in MCF-7 cell lysate.

Western Blot Analysis: 1 µg/mL of this antibody detected LAMP-3 in MCF-7 cell lysate.

Purified mouse monoclonal IgG2bκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Format: Purified
Protein G Purified

Control
MCF-7 cell lysate

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

100

mouse

purified immunoglobulin

primary antibodies

16H11.2, monoclonal

human

immunohistochemistry: suitable
western blot: suitable

IgG2bκ

NP_055213

Q9UQV4

wet ice

human ... LAMP3(27074)

~65 kDa observed.
The calculated molecular weight of this protein is 41 kDa, but has been observed at ~65-90 kDa due to glycosolation (de Saint-Vis, B., et al. (1998). Immunity. 9(3):325-336).

Stable for 1 year at 2-8°C from date of receipt.

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

12 - Non Combustible Liquids

WGK 1