Anti-LIM-3 Antibody

32160702

NA.41

Recognizes LIM-3. This antibody does not appear to cross react with gsh-4 on tissue sections but may cross react in Western blot and immunoprecipitation.

C-terminal portion of mouse LIM-3 protein.

Research Category
Neuroscience
Immunohistochemistry: 1:250-1:1,000 on paraffin embedded sections. 1:3,000-1:6,000 on frozen sections. 1:4,000-1:8,000 on whole mounts. Recommended fixative MEMFA.

Immunocytochemistry: 1:200-1:500

Western blot: 1:2,500-1:5,000

Immunoprecipitation: 1:200

This antibody will work well in immunofluorescence. Use a secondary antibody conjugated to biotin and then streptavidin-conjugated to fluorochrome.

Optimal working dilutions must be determined by the end user.
Research Sub Category
Developmental Neuroscience

Neuronal & Glial Markers
Anti-LIM-3 Antibody is an antibody against LIM-3 for use in IP, WB, IC, IH, IH(P).

Purified immunoglobulin. Liquid in 0.02M Phosphate buffer, 0.25M NaCl with 0.1% sodium azide.
Format: Purified

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

rabbit

100

purified immunoglobulin

primary antibodies

polyclonal

frog, mouse, fish, human

Chemicon^®

immunocytochemistry: suitable
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
immunoprecipitation (IP): suitable
western blot: suitable

NM_014945.2

O94929

wet ice

human ... ABLIM3(22885)

Maintain at 2-8°C in undiluted aliquots for up to 6 months.

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

10 - Combustible liquids

WGK 2