Anti-MLN64/STARD3 Antibody, clone 2BE-2F4

32160702

NA.41

MLN64/StARD3 (also known as es64, StAR-related lipid transfer protein 3, Metastatic lymph node gene 64 protein, Metastatic lymph node protein 64, StARD3, START domain-containing protein 3, Steroidogenic acute regulatory protein related; UniProt Q14849) is encoded by the MLN64 (or CAB1 & STARD3) gene in human (NCBI Gene ID 10948). MLN64/StARD3 is a late endosome (LE) multitransmembrane protein that plays an important role in intracellular cholesterol transport and steroidogenesis. In addition to binding cholesterol via its C-terminal STAR domain, MLN64/StARD3 is reported to interact with ER-anchored VAP proteins via a conserved motif called "two phenylalanines in an acidic tract" or FFAT, thereby forming an LE-ER tethering complex at the inter-organelle membrane contacts sites (MCSs), allowing heterologous membrane apposition and the exchange of metabolites (PMID 23709693 and 24105263). Clone 2BE-2F4 is also known as 2BE2F4 and mAbMLN64-Ct, it detects an epitope within the c-terminal region encompassing the last 20% of MLN64 sequence.

Ovalbumin-conjugated linear peptide corresponding to human MLN64/STARD3 near the C-terminus.
Epitope: Near C-terminus

Research Category
Signaling
Research Sub Category
Signaling Neuroscience
Immunocytochemistry Analysis: A representative lot detected MLN64/STARD3 in MCF7/MLN64 cells (Alpy, F., et al. (2001). J. Biol. Chem. 276:4261-4269).
Immunocytochemistry Analysis: A representative lot detected MLN64/STARD3 in BT-474, MCF7, and transfected COS-1 cells (Lutz-Moog, C., et al. (1997). Int. J. Cancer. 71:183-191).
Immunohistochemistry Analysis: A representative lot detected MLN64/STARD3 in human breast tissue (Lutz-Moog, C., et al. (1997). Int. J. Cancer. 71:183-191).
Electron Microscopy Analysis: A representative lot detected MLN64/STARD3 in HeLa cells (Holtta-Vuori, M., et al. (2005). Molecular Biology of the Cell. 16:3873-3886).
Western Blotting Analysis: A representative lot detected MLN64/STARD3 in COS-1 cell lysate (Watari, H., et al. (1997). Proc. Natl. Acad. Sci. USA. 94:8462-8467).
Western Blotting Analysis: A representative lot detected MLN64/STARD3 in various human cell lines (Lutz-Moog, C., et al. (1997). Int. J. Cancer. 71:183-191).
Anti-MLN64/STARD3 Antibody, clone 2BE-2F4 is an antibody against MLN64/STARD3 for use in Western Blotting and Immunocytochemistry, Immunohistochemistry, Electron Microscopy.

Evaluated by Western Blotting in SK-OV-3 cell lysate.

Western Blotting Analysis: A 1:2,000 dilution of this antibody detected MLN64/STARD3 in 10 µg of SK-OV-3 cell lysate.

Unpurified
Mouse monoclonal IgG1κ ascites with 0.05% sodium azide.

Concentration: Please refer to lot specific datasheet.

100

mouse

ascites fluid

primary antibodies

2BE-2F4, monoclonal

human

electron microscopy: suitable
immunocytochemistry: suitable
immunohistochemistry: suitable
western blot: suitable

IgG1κ

NP_001159409

Q14849

dry ice

~50 kDa observed. This protein has isoforms at ~48 and ~49 kDa, which are covered by this antigen.

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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