Anti-NuMA1 Antibody, clone AD6-1

32160702

NA.41

Nuclear mitotic apparatus protein 1 (UniProt: Q14980; also known as NuMA protein, Nuclear matrix protein-22, NMP-22, SP-H antigen) is encoded by the NUMA1 (also known as NMP22, NUMA) gene (Gene ID: 4926) in human. NMP-22 is a homodimeric nuclear protein that is highly abundant component of the nuclear matrix where it may serve a non-mitotic structural role and occupies the majority if the nuclear volume. It is required for maintenance and establishment of the mitotic spindle poles during symmetric cell divisions, functioning as a tether linking bulk microtubules of the spindle to centrosomes. It is also considered to be essential or proper alignment of the mitotic spindle during asymmetric cell divisions. NMP-22 resides in the nuclear matrix during interphase and then dissociates from condensing chromosomes during early prophase and relocates to the spindle poles via dynein/dynamin association and it remains there until the anaphase onset. As mitosis progresses it re-associates with telophase chromosomes very early during nuclear reformation, before substantial accumulation of lamins on chromosomal surfaces is evident. NMP-22 is phosphorylated in the C-terminal tail during mitosis, most likely by CDK1 and phosphorylation increases its solubility and promotes association with dynein and subsequent translocation to the spindle poles. O-glycosylation during cytokinesis at sites identical or close to phosphorylation sites interferes with the phosphorylation status of NMP-22.

Clone AD6-1 detects NuMA1 in human and mouse cells. It targets an epitope within 181 amino acids from the C-terminal region.

A recombinant fragment corresponding to 181 amino acids from the C-terminal region of human Nuclear mitotic apparatus protein 1 (NuMA1).

Western Blotting Analysis: 0.5 µg/mL from a representative lot detected NUMA1 in 10 µg of NIH/3T3 cell lysate.

Immunocytochemistry Analysis: A 1:50 dilution from a representative lot detected NUMA1 in HepG2 cells.

Immunocytochemistry Analysis: A representative lot detected NUMA1 in Immunocytochemistry applications (Carminati, M., et. al. (2016). Nat Struct Mol Biol. 23(2):155-63; Gallini, S., et. al. (2016). Curr Biol. 26(4):458-69).

Western Blotting Analysis: A representative lot detected NUMA1 in Western Blotting applications (Carminati, M., et. al. (2016). Nat Struct Mol Biol. 23(2):155-63; Gallini, S., et. al. (2016). Curr Biol. 26(4):458-69).

Research Category
Epigenetics & Nuclear Function
Anti-NuMA1, clone AD6-1 Antibody, Cat. No. MABE1807, is a highly specific mouse monoclonal antibody that targets NUMA1 and has been tested in Immunocytochemistry and Western Blotting.

Evaluated by Western Blotting in HeLa cell lysate.

Western Blotting Analysis: 0.5 µg/mL of this antibody detected NUMA1 in 10 µg of HeLa cell lysate.

Protein G purified
Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Format: Purified

Concentration: Please refer to lot specific datasheet.

100

mouse

purified antibody

primary antibodies

AD6-1, monoclonal

human, mouse

antibody small pack of 25 μg

immunocytochemistry: suitable
western blot: suitable

IgG1κ

NP_001273490

Q14980-1

ambient

human ... NUMA1(4926)

~250 kDa observed; 238.26 kDa calculated. Uncharacterized bands may be observed in some lysate(s).

Stable for 1 year at 2-8°C from date of receipt.

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.