Anti-Prodynorphin Antibody

32160702

NA.41

ProDynorphin, rat.

Synthetic peptide from rat ProDynorphin.

Anti-Prodynorphin Antibody is an antibody against Prodynorphin for use in IH.
Immunohistochemistry: 1:500

Optimal working dilutions must be determined by the end user.

APPLICATION NOTES FOR AB5519

IMMUNOHISTOCHEMISTRY

Perfusion: 1) Ca2+-free Tyrode′s solution followed by 2) paraformaldehyde-picric acid fixative and 3) 10% sucrose in PBS as a cryo-protectant. Tissues were rapidly dissected out and stored overnight in 0.1 M phosphate buffer (pH 7.4) containing 10% sucrose.

Slide-mounted tissue sections were incubated with blocking buffer for 1 hour at room temperature. Primary antibody was diluted in blocking buffer to the appropriate working dilution. Blocking buffer was removed and the slides were then incubated at 2-8°C for 18-24 hours with AB5519. After rinsing in PBS 3 times sections were incubated for 60 minutes at room temperature with Cy3-conjugated secondary antibodies. After mounting in a mixture of PBS and glycerol (1:3) containing 0.1% p-phenylenediamine, sections were examined with a Nikon Microphot-SA epifluorescence microscope.

Affinity purified Guinea pig antibody in PBS containing 0.05% sodium azide.

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

100

guinea pig

affinity isolated antibody

primary antibodies

polyclonal

affinity chromatography

rat

Chemicon^®

immunohistochemistry: suitable

NM_024411.2

P01213

dry ice

human ... PDYN(5173)

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

10 - Combustible liquids

WGK 2