Anti-SUFU Antibody, clone 15G3.1

32160702

NA.41

Suppressor of fused homolog (UniProt: Q9UMX1; also known as SUFUH) is encoded by the SUFU (also known as UNQ650/PRO1280) gene (Gene ID: 51684) gene in human. SUFUH acts as a negative regulator in the hedgehog signaling pathway that is ubiquitously distributed in adult tissues. Three isoforms of SUFUH have been described that are produced by alternative splicing. Isoform 1 is detected in fetal brain, lung, kidney and testis and isoform 2 is detected in fetal testis, and at much lower levels in fetal brain, lung and kidney. SUFUH is shown to down-regulate Gli1-and Gli2-mediated transactivation of target genes. Binding of SUFUH sequesters Gli proteins in the cytoplasm. It is a part of a corepressor complex that acts on DNA-bound Gli1 and may link Gli1 to beta-transducin repeat containing E3 Ubiquitin Protein Ligase (BTRC) and facilitate its proteasomal degradation. SUFUH regulates the formation of either the repressor form (Gli3R) or the activator form (Gli3A) of the full length form of GLi3 (Gli3FL). When complexed with SUFUH, Gli3FL remains in the cytoplasm and is maintained in a neutral state. Mutations in SUFU gene are known to cause medulloblastoma.

Clone 15G3.1 detects Suppressor of fused homolog (SUFU) in human and murine cells. It targets an epitope within 177 amino acids from the C-terminal half.

GST/His-tagged recombinant fragment corresponding to 177 amino acids from the C-terminal half of human Suppressor of fused homolog (SUFU).

Research Category
Signaling
Immunohistochemistry Analysis: A 1:50 dilution from a representative lot detected SUFU in human cerebellum, human testis, human pancreas, and human skin tissues.

Anti-SUFU, clone 15G3.1, Cat. No. MABS1883, is a mouse monoclonal antibdoy that detects Suppressor of fused homolog (SUFU) and is tested for use in Immunohistochemistry (Paraffin) and Western Blotting.

Evaluated by Western Blotting in NIH-3T3 cell lysate.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected SUFU in NIH-3T3 cell lysate.

Protein G purified
Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Format: Purified

Concentration: Please refer to lot specific datasheet.

100

mouse

purified immunoglobulin

primary antibodies

15G3.1, monoclonal

mouse, human

immunohistochemistry: suitable (paraffin)
western blot: suitable

IgG1κ

NP_057253.2

Q9UMX1

ambient

human ... SUFU(51684)

~54 kDa observed; 53.95 kDa calculated. Uncharacterized bands may be observed in some lysate(s).

Stable for 1 year at 2-8°C from date of receipt.

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