Anti-TorsinB Antibody

32160702

NA.41

TorsinB. The antibody reacts with the ~38 kDa protein.

Synthetic peptide from torsinB.

Detect TorsinB using this Anti-TorsinB Antibody validated for use in IP, WB & IC.
Western blot: 1:100-1:200. The antibody reacts with the ~38 kDa protein. The suggested blocking buffer is TBS with 10% milk and 0.05% Tween-20. The suggested dilution buffer is TBS with 1% milk and 0.05% Tween-20. Note: TorsinB is a glycoprotein which can be deglycosylated by either EndoH or PNGase treatment.
Immunocytochemistry: 1:100 using rhodamine conjugated goat anti-rabbit secondary antibody. Suggested fixative is 4% paraformaldehyde fixed (37°C for 20 minutes) cells. Suggested blocking solution should contain 10% goat serum. Suggested dilution buffer is PBS containing 1% BSA.
Immunoprecipitation. Recommended antibody amount is 5-7 mL of rabbit serum. The preferred tissue/cell lysis buffer is RIPA. Final reaction volume of 1000 mL. Final total protein concentration in reaction mix: 0.1-0.5 mg/mL. Suggested incubation time is 16-18 hours at 2-8°C. Sugggested capture is Protein A/G.

Optimal working dilutions must be determined by end user.

Maintain at frozen at -20°C in undiluted aliquots for up to 6 months after date of receipt. Avoid repeated freeze/thaw cycles.

Control
Western blot: brain and liver. Immunocytochemistry: SH-SY5Y cells.

rabbit

100

serum

primary antibodies

polyclonal

rat, mouse, human

Chemicon^®

immunocytochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable

NM_014506.1

O14657

dry ice

human ... TOR1B(27348)

Serum

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

11 - Combustible Solids

WGK 1