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Anti-TRRAP Antibody, clone 2TRR-1B3
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产品编号: | 4155041 |
规格: | ascites fluid, clone 2TRR-1B3, from mouse |
包装规格: | 100 μL |
产品类别: | 进口试剂 |
品牌: | Sigma-Aldrich |
优惠价: | 立即咨询 |
产品别名
Anti-TRRAP Antibody, clone 2TRR-1B3
Transformation/transcription domain-associated protein, 350/400 kDa PCAF-associated factor, PAF350/400, STAF40, TR-AP, Tra1 homolog
基本信息
eCl@ss | 32160702 |
General description【一般描述】 | Transformation/transcription domain-associated protein (UniProt Q9Y4A5; also known as 350/400 kDa PCAF-associated factor, PAF350/400, STAF40, TR-AP, Tra1 homolog) is encoded by the TRRAP (also known as PAF400) gene (Gene ID 8295) in human. Transformation/transcription domain-associated protein (TRRAP) is a cofactor component shared by several histone acetyltransferase (HAT) complexes, including SAGA (Spt-Ada-Gcn5-acetyltransferase), PCAF (P300/CBP-associated factor), and Tip60. TRRAP is an essential component of the histone epigenetic modifying mechinery that plays an important role in regulating cell-cycle progression. Trrap gene-knockdown or protein downregulation causes cell and mouse lethality, impaired spindle checkpoint and differentiation. |
Specificity【特异性】 | The immunogen sequence is present in both spliced isoforms of human TRRAP reported by UniProt (Q9Y4A5). |
Immunogen【免疫原】 | Epitope: N-terminal to nuclear localization signal in the TP53-interacting region. Ovalbumin-conjugated linear peptide corresponding to a sequence from the TP53-interacting region of human TRRAP. |
Application【应用】 | Research Category Epigenetics & Nuclear Function Anti-TRRAP Antibody, clone 2TRR-1B3 is an antibody against TRRAP for use in Western Blotting, Immunoprecipitation. Immunoprecipitation Analysis: 10 µL from a representative lot immunoprecipitated TRRAP from 1 mL (4.5 mg/mL) of HeLa nuclear extract (Courtesy of Dr. Mustapha OULAD-ABDELGHANI, IGBMC, France). Immunoprecipitation Analysis: A representative lot immunodepleted TRRAP using nonhomologous end-joining/NHEJ-competent protein extracts prepared from human AAH-1 lymphoblastoid cells (Robert, F., et al. (2006). Mol. Cell. Biol. 26(2):402-412). Research Sub Category Histone Modifying Proteins |
Quality【质量】 | Evalulated by Western Blotting in HeLa nuclear extract. Western Blotting Analysis: A 1:1,000 dilution of this antibody detected TRRAP in 10 µg of HeLa nuclear extract. |
Physical form【外形】 | Unpurified Mouse monoclonal IgG2aκ ascites without preservatives. |
Other Notes【其他说明】 | Concentration: Please refer to lot specific datasheet. |
产品性质
Quality Level【质量水平】 | 100 |
biological source【生物来源】 | mouse |
antibody form【抗体形式】 | ascites fluid |
antibody product type | primary antibodies |
clone【克隆】 | 2TRR-1B3, monoclonal |
species reactivity | human |
technique(s) | immunoprecipitation (IP): suitable western blot: suitable |
isotype【同位素/亚型】 | IgG2aκ |
NCBI accession no.【NCBI登记号】 | NP_001231509 |
UniProt accession no.【UniProt登记号】 | Q9Y4A5 |
shipped in【运输】 | dry ice |
产品说明
Target description【目标描述】 | ~400 kDa observed. 437.6 kDa (isoform 1) and 434.4 kDa (isoform 2) calculated. Uncharacterized band(s) may appear in some lysates. |
Storage and Stability【储存及稳定性】 | Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. |
Disclaimer【免责声明】 | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
安全信息
Storage Class Code【储存分类代码】 | 12 - Non Combustible Liquids |
WGK | WGK 1 |