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Anti-TUC-4 Protein Antibody
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产品别名
Anti-TUC-4 Protein Antibody
ULIP-1 Protein, CRMP-4, DRP-3, Dihydropyrimidinase-like 3
基本信息
eCl@ss | 32160702 |
NACRES | NA.41 |
General description【一般描述】 | TUC (TOAD [Turned On After Division]/ Ulip/CRMP) is a family of proteins that have emerged as strong candidates for a role in neuronal growth cone signaling. The TUC-4 family members reach their highest expression levels in early post-mitotic neurons during their peak periods of axonal growth. TUC-4 is not expressed in neural progenitors or adult neurons (with minor exception). Data suggests that TUC-4 plays a role in axonal outgrowth, pathfinding and possibly in the process that permits growing axons to choose proper routes and targets (Quinn, 1999). The TUC-4 protein is expressed primarily within the cytoplasm of postmitotic neurons as they begin their migration out of the ventricular zone into the developing cortical plate (Minturn, 1995a). TUC-4 is expressed in neuronal cell bodies, neurites, and along the most distal regions of neuronal growth cones, including the lamellipodia and filopodia (Minturn, 1995b). Expression of TUC-4 is observed by day E12 in rats coincident with the expression of the early neuronal marker class III beta-tubulin (Quinn, 1999). TUC-4 expression is rarely observed in neuronal cells expressing the mitotic marker PCNA (Minturn, 1995a). The expression of TUC-4 has been shown to be upregulated in response to NGF in rats (Minturn, 1995b) and in response to retinoic acid in a human neuroblastoma cell line (SMS-KCNR; Gaetano, 1997). |
Specificity【特异性】 | Specifically recognizes the TUC-4 (Ulip-1/CRMP-4/DRP-3) splice variants, TUC-4a (64 kDa) and the N-terminally extended TUC-4b (75 kDa) (Quinn, 2003). AB5454 has been reported to label early postmitotic neurons in prenatal rat brain. Labeling is observed primarily within the cytoplasm along the most distal regions of neuronal growth cones including lamellipodia and filopodia. The antibody is not reactive with the proteins TUC-1 (Ulip-3/CRMP-1), TUC-2 (Ulip-2/CRMP-2) or TUC-3 (Ulip-4/CRMP-3). It does not label neural progenitors or radial glia. |
Immunogen【免疫原】 | Synthetic peptide corresponding to amino acids 499 to 511 of TUC-4a and amino acids 612-624 of TUC-4b (YDGPVFDLTTTPK) |
Application【应用】 | Immunohistochemistry: 1:1,000-1:5,000 using PFA (DAB detection). Immunocytochemistry: 1:1,000-1:5,000 (DAB detection). Western blot: 1:2,500-1:25,000 using alkaline phosphatase. Immunoprecipitation Optimal working dilutions must be determined by end user. Anti-TUC-4 Protein Antibody detects level of TUC-4 Protein & has been published & validated for use in IC, IH, IP & WB. |
Physical form【外形】 | Unpurified Rabbit Serum. Liquid containing 0.2% sodium azide. |
Analysis Note【分析说明】 | Control POSITIVE CONTROL: Fetal brain/spinal cord tissue. Human neuroblastoma cell line SMS-KCNR or rat PC12 cells. |
Other Notes【其他说明】 | Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration. |
Legal Information【法律信息】 | CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany |
产品性质
biological source【生物来源】 | rabbit |
Quality Level【质量水平】 | 100 |
antibody form【抗体形式】 | serum |
antibody product type | primary antibodies |
clone【克隆】 | polyclonal |
species reactivity | rat, monkey, feline, mouse, human |
manufacturer/tradename | Chemicon® |
technique(s) | immunocytochemistry: suitable immunohistochemistry: suitable immunoprecipitation (IP): suitable western blot: suitable |
NCBI accession no.【NCBI登记号】 | NM_001387.2 |
UniProt accession no.【UniProt登记号】 | Q14195 |
shipped in【运输】 | dry ice |
Gene Information | human ... DPYSL3(1809) |
产品说明
Target description【目标描述】 | 64 & 74 kDa |
【免责声明】 | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
安全信息
Storage Class Code【储存分类代码】 | 12 - Non Combustible Liquids |
WGK | WGK 1 |