Biotin-X-NHS, Water-Soluble

C₂₀H₂₉N₄O₉S₂ · Na

556.59

Water solubility is extremely convenient, eliminating the need to dissolve the reagent in an organic solvent such as dimethylformamide (DMF) and dimethylsulfoxide (DMSO) prior to use.
Readily couples biotin to primary amino groups of proteins under mild alkaline conditions. Ideally suited for labeling antibodies, hormones, or other polypeptide molecules containing lysine residues. Extended spacer arm provides maximum accessibility of biotin for avidin conjugates.

Toxicity: Standard Handling (A)

Unstable in aqueous solution; reconstitute just prior to use. Following reconstitution in DMSO, Aliquot and freeze (-20°C). DMSO stock solutions are stable for up to 1 month -20°C.

Gretch, D.R., et al. 1987. Anal. Biochem.163, 270.

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

100

≥94% (HPLC)

solid

Calbiochem^®

OK to freeze
desiccated

off-white to tan

DMSO: 13 mg/mL
distilled water: 5.5 mg/mL

ambient

2-8℃

50 mg in Plastic ampoule

11 - Combustible Solids

WGK 3