Cathepsin K, His·Tag^®, Human, Recombinant, E. coli

3.4.22.38 ( BRENDA | IUBMB )

Cathepsin K is a member of the papain superfamily of cysteine proteinases and plays an important role in osteoclast-mediated bone resorption and collagen degradation. It is primarily expressed by osteoclasts. Recently reported to also be expressed in fibroblasts. Irregularities in activity contribute to osteopenic disorders such as osteoporosis and pycnodysostosis. Contains a C-terminal His•Tag.
Note: 1 mU = 1 milliunit.
Cathepsin K is a member of the papain superfamily of cysteine proteinases and plays an important role in osteoclast-mediated bone resorption and collagen degradation. It is primarily expressed by osteoclasts and recent studies also suggest its expression in fibroblasts. Irregularities in cathepsin K activity, either too high or too low, contribute to osteopenic disorders such as osteoporosis and pycnodysostosis. This recombinant enzyme contains a C-terminal His•Tag sequence.

Toxicity: Standard Handling (A)

One unit is defined as the amount of enzyme that will hydrolyze 0.4 µmol Z-FR-AMC per min at 37°C, pH 5.5.

In 50 mM NaOAC, pH 5.5. The activation was stopped by addition of 10 mM MMTS, which can be removed under reducing conditions.

Following initial thaw, aliquot and freeze (-70°C).

Delaisse, J.M., et al. 2003. Microsc. Res. Tech.61, 504.
Dodds, R.A. 2003. Cell Biochem. Funct.21, 231.
Everts, V., et al. 2003. Calcif. Tissue Int.73, 380.
Hou, W.S., et al. 2003. Biol. Chem.384, 891.

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
HIS TAG is a registered trademark of Merck KGaA, Darmstadt, Germany

100

expressed in E. coli

≥95% (SDS-PAGE)

liquid

≥900 mU/mg protein

Calbiochem^®

OK to freeze
avoid repeated freeze/thaw cycles

wet ice

−70℃

25 μg in Plastic ampoule
Please refer to vial label for lot-specific concentration.

12 - Non Combustible Liquids

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