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ChIPAb+ Histone H4-ChIP Validated Antibody and Primer Set, rabbit monoclonal

产品编号:4155649
规格:from rabbit
包装规格:25 ASSAYS
产品类别:进口试剂
品牌:Sigma-Aldrich
优惠价:立即咨询
产品价格
产品编号包装单位单价(元)国内现货国外库存询价单
415564925 ASSAYS6850
产品别名

ChIPAb+ Histone H4-ChIP Validated Antibody and Primer Set, rabbit monoclonal

H4, Histone H4, H4 histone family, member A, histone 1, H4a, histone cluster 1, H4a

基本信息
eCl@ss
32160702
NACRES
NA.32
General description【一般描述】
All ChIPAb+ antibodies are individually validated for chromatin precipitation, every lot, every time. Each ChIPAb+ antibody set includes control primers (tested every lot by qPCR) to biologically validate your IP results in a locus-specific context. The qPCR protocol and primer sequences are provided, allowing researchers to validate ChIP protocols when using our antibody in their chromatin context. Each set also includes a negative control antibody to ensure specificity of the ChIP reaction.
The ChIPAb+ Histone H4 set includes the Histone H4 antibody, a negative control antibody (normal rabbit IgG), and qPCR primers which amplify a 110 bp region of human β-Globin. The Histone H4 and negative control antibodies are supplied in a scalable "per ChIP" reaction size and can be used to functionally validate the precipitation of Histone H4-associated chromatin.
Specificity【特异性】
Wide range of cross-reactivity expected based on sequence homology.
Immunogen【免疫原】
Epitope: a.a 25-28
KLH-conjugated synthetic peptide corres-ponding to amino acids 17-28 of Histone H4.
Application【应用】
Chromatin Immunoprecipitation:
Sonicated chromatin prepared from HeLa cells (1 X 106 cell equivalents per IP) were subjected to chromatin immunoprecipitation using 2 µL of either normal rabbit IgG or 2 µL of Anti-Histone H4 antibody and the Magna ChIP A Kit (Cat. # 17-610).
Successful immunoprecipitation of total Histone H4 associated DNA fragments was verified by qPCR using ChIP Primers, β-Globin, as well as GAPDH promoter Control Primers, (Please see figures).
Data is presented as percent input of each IP sample relative to input chromatin for each amplicon and ChIP sample as indicated.

Please refer to the EZ-Magna ChIP A (Cat. # 17-408) or EZ-ChIP (Cat. # 17-371) protocol for experimental details.

Western Blot Analysis:
Representative Lot Data.
HeLa Acid Extract (Lane 1 and 3), HeLa Acid Extract from sodium butyrate treated cells (Lane 2), HeLa Acid Extract from colcemid treated cells (Lane 4) and recombinant histone H4 (Lane 5) were resolved by electrophoresis, transferred to PVDF membranes and probed with Anti-Histone H4 (1:1,000 dilution).
Proteins were visualized using a Donkey anti-rabbit IgG conjugated to HRP and visualized using a chemiluminescence detection system. (Please see figures).

This ChIPAb+ Histone H4 -ChIP Validated Antibody & Primer Set conveniently includes the antibody & the specific control PCR primers.
Quality【质量】
Chromatin Immunoprecipitation:
Sonicated chromatin prepared from HeLa cells (1 X 106 cell equivalents per IP) were subjected to chromatin immunoprecipitation using 2 µL of either normal rabbit IgG or 2 µL of Anti-Histone H4 antibody and the Magna ChIP® A Kit (Cat. #17-610). Successful immunoprecipitation of total Histone H4 associated DNA fragments was verified by qPCR using ChIP Primers, β-Globin (Please see figures).

Please refer to the EZ-Magna ChIP A (Cat. # 17-408) or EZ-ChIP (Cat. # 17-371) protocol for experimental details.


Physical form【外形】
Format: Purified
Anti-Histone H4 (rabbit monoclonal IgG). One vial containing 50 µL of protein A purified rabbit monoclonal IgG supernatant in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide before addition of glycerol to 40%. Store at -20°C.

Normal Rabbit IgG. One vial containing 75 µg of normal rabbit IgG in 75 µL storage buffer. Store at -20°C.

ChIP Primers, β-Globin. One vial containing 75 μL of 5 μM of each primer specific for human β-Globin. Store at -20°C.
FOR: AGG ACA GGT ACG GCT GTC ATC
REV: TTT ATG CCC AGC CCT GGC TC


Protein A purified
Analysis Note【分析说明】
Control
Includes negative control antibody (normal rabbit IgG) and primers specific for human β-Globin.
Legal Information【法律信息】
MAGNA CHIP is a registered trademark of Merck KGaA, Darmstadt, Germany
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
产品性质
biological source【生物来源】
rabbit
Quality Level【质量水平】
100
clone【克隆】
monoclonal
species reactivity
human
manufacturer/tradename
ChIPAb+
Upstate®
technique(s)
ChIP: suitable
immunoprecipitation (IP): suitable
western blot: suitable
NCBI accession no.【NCBI登记号】
NP_001029249
UniProt accession no.【UniProt登记号】
P62805
shipped in【运输】
dry ice
产品说明
Target description【目标描述】
~11 kDa
【免责声明】
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
安全信息
Storage Class Code【储存分类代码】
10 - Combustible liquids
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