DG 18 (Dichloran glycerol chloramphenicol) agar (base)

ISO

200

FDA

medium granules (dehydrated (DCM))

GranuCult^®

microbiological culture: suitable

5.4-5.8 (25 ℃ in H₂O)

food and beverages
microbiology

15-25℃

molds
yeasts

GranuCult is our new brand name for our existing granulated dehydrated culture media which apply to new EN ISO 11133:2014 and various other ISO standards and FDA-BAM / USDA-FSIS methods for testing of food and water samples. All these media are currently being updated to be fully compliant to the latest version of these standards and regulations with respect to composition and quality control and will be available shortly.

Appearance (clearness): clear to slightly opalescent
Appearance (colour): yellowish
pH-value (25 °C): 5.4 - 5.8
Growth promotion test in accordance with the current version of DIN EN ISO 11133.
Inoculum on reference medium (Saccharomyces cerevisiae ATCC 9763 (WDCM 00058)):
Colony count (Saccharomyces cerevisiae ATCC 9763 (WDCM 00058)):
Recovery on test medium (Saccharomyces cerevisiae ATCC 9763 (WDCM 00058)): ≥ 50 %
Inoculum on reference medium (Wallemia sebi ATCC 42694 (WDCM00182)):
Colony count (Wallemia sebi ATCC 42694 (WDCM00182)):
Recovery on test medium (Wallemia sebi ATCC 42694 (WDCM00182)): ≥ 50 %
Inoculum on reference medium (Aspergillus caesiellus ATCC 42693 (WDCM00183)):
Colony count (Aspergillus caesiellus ATCC 42693 (WDCM00183)):
Recovery on test medium (Aspergillus caesiellus ATCC 42693 (WDCM00183)): ≥ 50 %
Inoculum on reference medium (Eurotium rubrum ATCC 42690 (WDCM00183)):
Colony count (Eurotium rubrum ATCC 42690 (WDCM00183)):
Recovery on test medium (Eurotium rubrum ATCC 42690 (WDCM00183)): ≥ 50 %
Growth (Escherichia coli ATCC 8739 (WDCM 00012)): no growth
Growth (Escherichia coli ATCC 25922 (WDCM 00013)): no growth
Growth (Bacillus subtilis ATCC 6633 (WDCM 00003)): no growth
Growth (Mucor racemosus ATCC 42647): good to very good
Growth (Penicillium cyclopium CMI 19759): good to very good
Growth (Rhizopus stolonifer ATCC 15441): good to very good
Incubation: 5 days at 25 ± 1 °C
A recovery rate of 50 % is equivalent to a productivity value of 0.5.
The indicated colony counts result from the sum of a triple determination.
Reference media: SABOURAUD dextrose agar

We offer two media types: the superior granulated GranuCult^® and the cost-efficient powdered NutriSelect^® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

11 - Combustible Solids

WGK 3