DNA Base Excision Repair Pathway Inhibitor-CAS 6960-45-8-Calbiochem

C₉H₆N₂O₄

206.15

MFCD00044720

A cell-permeable, potent, specific, and nontoxic inhibitor of the DNA repair enzyme, APE1 (human apurinic/apyrimidinic endonuclease; IC₅₀ = ~3 µM). Shown to target the APE1 active site and inhibit its 3′-phosphodiesterase and 3′-phosphatase activities. Exhibits minimal effects on endonuclease IV, BamH1 restriction endonuclease, or topoisomerase I even at concentration as high as 100 µM. Also shown to potentiate the cytotoxicity of several DNA damaging agents, such as MMS, temozolomide, Zeocin, and H₂O₂ in HeLa, HT1080, and MDA-MB-231 cells.
A cell-permeable, potent, specific, and nontoxic inhibitor of the DNA repair enzyme, APE1 (human apurinic/apyrimidinic endonuclease; IC₅₀ ~3 µM). Shown to target the APE1 active site and inhibit its 3′-phosphodiesterase and 3′-phosphatase activities. Exhibits minimal effects on endonuclease IV, BamH1 restriction endonuclease, or topoisomerase I even at concentration as high as 100 µM. Also shown to potentiate the cytotoxicity of several DNA damaging agents, such as MMS, temozolomide, Zeocin, and H₂O₂ in HeLa, HT1080, and MDA-MB-231 cells.

Cell permeable: yes
Primary Target
APE 1
Product does not compete with ATP.
Reversible: no
Target IC₅₀: ~3 µM against APE1 (human apurinic/apyrimidinic endonuclease

Toxicity: Irritant (B)

Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.

Madhusudan, S., et al. 2005. Nucl. Acids Res.33, 4711.

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

100

≥98% (HPLC)

solid

Calbiochem^®

OK to freeze
protect from light

tan

DMSO: 5 mg/mL

ambient

2-8℃

1S/C9H6N2O4/c12-9(13)6-4-5-2-1-3-7(11(14)15)8(5)10-6/h1-4,10H,(H,12,13)/p-1

BIUCOFQROHIAEO-UHFFFAOYSA-M

25 mg in Plastic ampoule
Packaged under inert gas

GHS08

Danger

H319 - H334

P261 - P264 - P280 - P284 - P304 + P340 + P312 - P305 + P351 + P338

Eye Irrit. 2 - Resp. Sens. 1

11 - Combustible Solids

WGK 3