Endo-β-Galactosidase, Bacteroides fragilis, Recombinant, E. coli

3.2.1.103 ( BRENDA | IUBMB )

Recombinant, Bacteroides fragilis endo-β-galactosidase expressed in E. coli. Endo-β-Galactosidase is an enzyme that hydrolyzes internal β-galactosidic linkages of oligosaccharides in poly-N-acetyl-lactosamine structures. This enzyme resembles the Escherichia freundii enzyme due to its specificity towards bovine corneal keratan sulphate, milk oligosaccharides and the glycolipids lacto-N-neotetraosylceramide and lacto-N-tetraosylceramide. Optimum pH: 5.8.
Recombinant, Bacteroides fragilis endo-β-galactosidase expressed in E. coli. Endo-β-Galactosidase is an enzyme that hydrolyzes internal β-galactosidic linkages of oligosaccharides in poly-N-acetyl-lactosamine structures. This enzyme resembles the Escherichia freundii enzyme due to its specificity towards bovine corneal keratan sulphate, milk oligosaccharides and the glycolipids lacto-N-neotetraosylceramide and lacto-N-tetraosylceramide.

Toxicity: Standard Handling (A)

One unit is defined as the amount of enzyme that will liberate one µmol reducing sugar from bovine corneal keratan sulfate per min at 37°C, pH 5.8.

Sterile-filtered in 20 mM Tris-HCl, pH 7.5.

There is no significant loss of activity if left at ambient temperature for several days. The enzyme is active for at least 5 days under the Recommended Reaction Conditions above.

Murata, T., et al. 2003. Eur. J. Biochem.270, 3709.
Scudder, P., et al. 1986. Eur. J. Biochem.157, 365.
Scudder, P., et al. 1984. J. Biol. Chem.259, 6586.
Scudder, P., et al. 1983. Biochem. J.213, 485.

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

100

expressed in E. coli

liquid

≥14 U/mL
≥140 units/mg protein

Calbiochem^®

OK to freeze

ambient

2-8℃

50 μL in Plastic ampoule

12 - Non Combustible Liquids

nwg