Epiregulin, Mouse, Recombinant

100

expressed in E. coli

≥97% (SDS-PAGE)

liquid

Calbiochem^®

OK to freeze

<1.0 EU/μg protein Endotoxin

ambient

−20℃

50 μg in Glass bottle

Recombinant, mouse epiregulin expressed in E. coli with an N-terminal methionine. Epiregulin is shown to inhibit epithelial tumor growth, able to stimulate growth of fibroblasts and other cell types, and has been shown to be an autocrine growth factor in human epidermal keratinocytes.

ED₅₀ = 0.3-1 ng/ml as measured in a cell proliferation assay using Balb/3T3 mouse embryonic fibroblasts.

Toxicity: Standard Handling (A)

Lyophilized from 0.2 µm sterile-filtered PBS, BSA.

Following reconstitution refrigerate (4°C) for short-term storage or aliquot and freeze (-20°C) for long-term storage. Stock solutions are stable for up to 1 month at 4°C or for up to 3 months at -20°C.
Reconstitute to 10 µg/ml in sterile PBS containing at least 0.1% human or bovine serum albumin.

Shirakata, Y., et al. 2000. J. Biol. Chem.275, 5748.
Shelly, M., et al. 1998. J. Biol. Chem.273, 10496.
Das, S.K., et al. 1997. Dev. Biol.190, 178.
Komurasaki, T., et al. 1997. Oncogene15, 2841.
Toyoda, H., et al. 1995. J. Biol. Chem.270, 7495.
Toyoda, H., et al. 1995. FEBS Lett.377, 403.

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

13 - Non Combustible Solids

WGK 3