Exo1-CAS 75541-83-2-Calbiochem

C₁₅H₁₂FNO₃

273.26

MFCD00028104

A cell-permeable methylanthranilate analog that reversibly blocks vesicular traffic from the ER to the Golgi in mammalian cells (IC₅₀ = 20 µM in BSC1 cells) by inducing tubulation and collapsing of the Golgi membrane and re-directing the traffic back to the ER. Acts as a potent and selective modifier of Golgi ARF1 GTPase activity. Unlike another membrane traffic inhibitor, Brefeldin A (BFA) (Cat. No. 203729), the effects appear to be limited to the Golgi, as no effects are detected on endocytic organelles, such as endosomes and trans-Golgi network (TGN). The inhibitory mechanism and target(s) appear to be different from those of BFA, thus making it a valuable alternative that complements BFA in membrane trafficking studies.
A cell-permeable methylanthranilate analog that reversibly inhibits vesicular traffic from ER to Golgi in mammalian cells (IC₅₀ = 20 µM in BSC1 cells) by inducing tubulation and collapsing of the Golgi membrane and redirecting the traffic back to ER. Acts as a potent and selective modifier of Golgi ARF1 GTPase activity. Unlike another membrane traffic inhibitor Brefeldin A (BFA; Cat. No. 203729), its effect seems to be limited to Golgi, as no effect can be detected on endocytic organelles, such as endosomes and TGN (trans-Golgi network). Its inhibitory mechanism and target(s) appear to be quite different from those of BFA and thus serves as a valuable alternative that complements BFA in membrane traffic studies.

Cell permeable: yes
Target IC₅₀: 20 µM inhibiting vesicular traffic from ER to Golgi in BSC1 cells
Product does not compete with ATP.
Primary Target
Golgi ARF1 GTPase
Reversible: yes

Toxicity: Harmful (C)

Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.

Feng. Y., et al. 2003. Proc. Natl. Acad. Sci. USA100, 6469.

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

100

≥95% (HPLC)

solid

Calbiochem^®

OK to freeze
protect from light

white

DMF: 25 mg/mL
DMSO: 25 mg/mL
ethanol: 5 mg/mL
methanol: 5 mg/mL

ambient

2-8℃

1S/C15H12FNO3/c1-20-15(19)12-4-2-3-5-13(12)17-14(18)10-6-8-11(16)9-7-10/h2-9H,1H3,(H,17,18)

KIAPWMKFHIKQOZ-UHFFFAOYSA-N

25 mg in Plastic ampoule
Packaged under inert gas

11 - Combustible Solids

WGK 3