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GC-RICH PCR System, dNTPack

产品编号:4156980
规格:suitable for PCR, hotstart: no, Difficult Templates/Specialty Enzymes PCR
包装规格:100 U
产品类别:进口试剂
品牌:Roche
优惠价:立即咨询
产品价格
产品编号包装单位单价(元)国内现货国外库存询价单
4156980100 U2710
产品别名

GC-RICH PCR System, dNTPack

GC-rich PCR

产品性质
usage【用途】
sufficient for ≤50 reactions
feature【特点】
Difficult Templates/Specialty Enzymes PCR
dNTPs included
hotstart: no
manufacturer/tradename
Roche
parameter【参数】
72 ℃ optimum reaction temp.
technique(s)
PCR: suitable
input
purified DNA
shipped in【运输】
dry ice
storage temp.【储存温度】
−20℃ (−15℃ to −25℃)
packaging【包装】
1 kit containing 6 components
基本信息
General description【一般描述】
The GC-RICH PCR System, dNTPack is composed of a special enzyme blend of thermostable Taq DNA Polymerase and Tgo DNA Polymerase, a thermostable enzyme with a proofreading (3′-5′ exonuclease) activity. This polymerase mixture by itself outperforms Taq DNA Polymerase in respect to yields, fidelity and specificity beside the possibility to amplify fragments up to 5 kb in length. The GC-RICH PCR reaction buffer in combination with the included GC-RICH resolution solution allows to amplify very efficiently difficult templates like GC-rich targets.
Application【应用】
The GC-RICH PCR System, a blend of Taq DNA Polymerase and a proofreading polymerase, enables amplification of templates that are difficult or impossible to amplify with other polymerases and other blends of polymerases. The enhanced processivity of the blend and the unique GC-RICH Resolution Solution combine to deliver superior performance – especially from problematic templates.
The GC-RICH PCR System, dNTPack may also be used in standard PCR applications, providing improved results (higher yield, higher accuracy) over Taq DNA Polymerase alone.
Features and Benefits【特点和优势】
  • Ease access to difficult templates:-
including GC-rich targets and repetitive sequences.
  • Reagents, the GC-RICH Resolution Solution and PCR Grade Water are provided.
  • Amplify DNA fragments up to 5 kb.
  • Cost-effective:-
use the convenient premixed solution of PCR grade NTPs.
Quality【质量】
The GC-RICH PCR System is function-tested, by amplifying a human 284 bp ApoE genomic fragment, using the GC-RICH PCR System protocols.
Unit Definition【单位定义】
Volume Activity: 2 U/μl
Preparation Note【制备说明】
Working concentration: The optimal enzyme concentration range from 0.5 to 5 U per assay. For a standard 50 μl PCR, we recommend using 2 U of the enzyme blend.
Other Notes【其他说明】
仅用于生命科学研究。不可用于诊断。
Legal Information【法律信息】
Use of this product is covered by US patent claims and corresponding patent claims outside the US. The purchase of this product includes a limited, non-transferable immunity from suit under the foregoing patent claims for using only this amount of product for the purchaser′s own internal research. No right under any other patent claim, no right to perform any patented method, and no right to perform commercial services of any kind, including without limitation reporting the results of purchaser′s activities for a fee or other commercial consideration, is conveyed expressly, by implication, or by estoppel. This product is for research use only. Diagnostic uses under Roche patent claims require a separate license from Roche. Further information on purchasing licenses may be obtained by contacting the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA.
Components【组分】
组份不可单独销售
GC-RICH Enzyme Mix, in storage buffer
GC-RICH PCR Reaction Buffer, with 7.5 mM MgCl2 and DMSO 5x concentrated
GC-RICH Resolution Solution 5 M
MgCl2 Stock Solution 25 mM
Water, PCR Grade
PCR Nucleotide Mix
安全信息
Hazard Statements
H412
Precautionary Statements
P273 - P501
Hazard Classifications【危险分类】
Aquatic Chronic 3
Storage Class Code【储存分类代码】
12 - Non Combustible Liquids
WGK
WGK 2
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