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Granzyme A, Human, Recombinant, E. coli
| 产品编号： | 4157254 |
| 规格： | Granzyme A, Human, Recombinant, E. coli, is a trypsin-like serine protease secreted by cytotoxic T cells and natural killer cells. Induces cell death through caspase-independent mechanism. |
| 包装规格： | 10 μG |
| 产品类别： | 进口试剂 |
| 品牌： | Sigma-Aldrich |
| 优惠价： | 立即咨询 |
产品别名
Granzyme A, Human, Recombinant, E. coli
GzmA
基本信息
| Enzyme Commission (EC) Number【酶学委员会(EC)编号】 | 3.4.21.78 ( BRENDA | IUBMB ) |
| General description【一般描述】 | Recombinant, human granzyme A expressed in E. coli. Granzyme A is a trypsin-like serine protease found in the cytotoxic granules and secreted by cytotoxic T lymphocytes (CTL) and natural killer (NK) cells. Granzyme A is a pro-apoptotic enzyme that induces cell death through a caspase-independent mechanism. Granzyme A-induced apoptosis results in single-stranded DNA nicking, nuclear fragmentation, chromatin condensation, loss of mitochondrial potential, and membrane blebbing. Granzyme A is also known to bind to and cleave nucleolin, activate the thrombin receptor on neuronal cells and astrocytes, cleave and activate interleukin-1β, and cleave putative HLA-associated protein II (PHAP II). Recombinant human Granzyme A expressed in E. coli. Recombinant, human granzyme A expressed in E. coli. Granzyme A is a trypsin-like serine protease secreted by cytotoxic T lymphocytes (CTL) and natural killer (NK) cells. A proapoptotic enzyme that induces cell death through caspase-independent mechanism, causing single-stranded DNA nicking and other apoptotic features including nuclear fragmentation, chromatin condensation, loss of mitochondrial potential, and membrane blebbing. Granzyme A is also known to bind to and cleave nucleolin and activate the thrombin receptor on neuronal cells and astrocytes. It also cleaves and activates interleukin-1β. Note: The M.W. is that of a homodimer. |
| Warning【警告】 | Toxicity: Standard Handling (A) |
| Unit Definition【单位定义】 | One unit is defined as the amount of enzyme that hydrolyzes 1 nmol Z-Lys-SBzl per min 25°C, pH 8.0. |
| Physical form【外形】 | In 520 mM NaCl, 50 mM MES, 10% glycerol, pH 6.0. |
| Other Notes【其他说明】 | Fan, Z., et al. 2002. Mol. Cell. Biol.22, 2810. Russell, J.H., and Ley, T. J. 2002. Annu. Rev. Immunol.20, 323. Beresford, P.J., et al. 2001. J. Biol. Chem.276, 43285. Simon, M.M., and Kramer, M.D. 1994. Methods Enzymol.244, 68. |
| Legal Information【法律信息】 | CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany |
产品性质
| Quality Level【质量水平】 | 100 |
| recombinant【重组】 | expressed in E. coli |
| form【形式】 | liquid |
| Specific Activity | ≥100 units/μg protein |
| manufacturer/tradename | Calbiochem® |
| storage condition【储存条件】 | OK to freeze avoid repeated freeze/thaw cycles |
| shipped in【运输】 | wet ice |
| storage temp.【储存温度】 | −70℃ |
| packaging【包装】 | 10 μg in Plastic ampoule |
安全信息
| Storage Class Code【储存分类代码】 | 10 - Combustible liquids |
| WGK | WGK 1 |
